FIGURE

Fig. 4

ID

ZDB-FIG-090408-28

Publication

Wilkinson et al., 2009 - Cep70 and Cep131 contribute to ciliogenesis in zebrafish embryos

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Fig. 4

Cilia in the otic vesicle, spine and pronephros at 30 h.p.f. (A-C) Spine of WT, cep70 and cep131 morphants, stained for acetylated tubulin (red, cilia) and gamma tubulin (green, basal bodies). The cilia of the central canal can be seen in between the two nerve tracts. Cilia are shortened in the morphant embryos but basal bodies remain intact. Pronephric ducts – arrows – are enlarged in morphant cep70 embryos (E) compared to WT (D) at 48 h.p.f. . (F-H) Cilia are also reduced in length in the pronephros of the morphants (G, H) compared to WT (F). However, like other tissues, basal bodies are present. Basal bodies are also present in surrounding tissues (inset). One WT cilium has been colored white for clarity. (I-K) Otic vesicles of WT, cep70 and cep131 morphants. Compass in the panels shows orientation: A = anterior, P = posterior, D = dorsal, V = ventral, L = left, R = right. Scale bars: 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-cep70 WT + MO1-cep131
Knockdown Reagents:	MO1-cep70 MO1-cep131
Observed In:	central canal ciliary basal body central canal cilium otic vesicle ciliary basal body otic vesicle kinocilium pronephric duct pronephric duct ciliary basal body pronephric duct cilium
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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Open Access.

Full text @ BMC Cell Biol.