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ZFIN ID: ZDB-FIG-090223-32
Jin et al., 2009 - Definitive hematopoietic stem/progenitor cells manifest distinct differentiation output in the zebrafish VDA and PBI. Development (Cambridge, England)   136(4):647-654 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 6 Inhibition of HSPC migration from the VDA to PBI blocks their differentiation into erythroid but not myeloid lineages. (A,B) Whole-mount in situ hybridization of runx1 expression in 30 hpf control MO injected vltm651 embryos (A) and sih MO injected vltm651 embryos (B). (C-H) Whole-mount in situ hybridization of cmyb expression in control MO-injected vltm651 embryos at 30 hpf (C), 3 dpf (E) and 4 dpf (G); sih MO injected vltm651 embryos at 30 hpf (D), 3 dpf (F) and 4 dpf (H). (I,J) Whole-mount in situ hybridization of βe1-globin expression in 4 dpf control MO injected vltm651 embryos (I) and sih MO-injected vltm651 embryos (J). (K,L) Whole-mount in situ hybridization of lyc expression in 4 dpf control MO-injected vltm651 embryos (K) and sih MO-injected vltm651 embryos (L). Insets are higher magnification (20x) views of the corresponding boxed regions (blue).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
hbae1.1 gata1am651/m651 control Day 4 post-vent region erythroid progenitor cell ISH
gata1am651/m651 + MO1-tnnt2a standard conditions Day 4 Not Detected post-vent region erythroid progenitor cell ISH
Day 4 Not Detected ventral wall of dorsal aorta erythroid progenitor cell ISH
lyz gata1am651/m651 control Day 4 ventral wall of dorsal aorta myeloid cell ISH
gata1am651/m651 + MO1-tnnt2a standard conditions Day 4 ventral wall of dorsal aorta myeloid cell ISH
myb gata1am651/m651 control Prim-15 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
Protruding-mouth post-vent region hematopoietic multipotent progenitor cell ISH
Day 4 post-vent region hematopoietic multipotent progenitor cell ISH
gata1am651/m651 + MO1-tnnt2a standard conditions Prim-15 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
Protruding-mouth ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
Day 4 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
runx1 gata1am651/m651 control Prim-15 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
gata1am651/m651 + MO1-tnnt2a standard conditions Prim-15 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gata1am651/m651 control Day 4 primitive hemopoiesis process quality, abnormal
gata1am651/m651 + MO1-tnnt2a standard conditions Protruding-mouth blood circulation arrested, abnormal
Protruding-mouth definitive hemopoiesis process quality, abnormal
Protruding-mouth hematopoietic multipotent progenitor cell mislocalised, abnormal
Protruding-mouth primitive hemopoiesis process quality, abnormal
Day 4 blood circulation arrested, abnormal
Day 4 blood island hematopoietic multipotent progenitor cell absent, abnormal
Day 4 definitive hemopoiesis process quality, abnormal
Day 4 erythrocyte differentiation process quality, abnormal
Day 4 erythroid progenitor cell absent, abnormal
Day 4 hematopoietic multipotent progenitor cell mislocalised, abnormal
Day 4 primitive hemopoiesis process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development