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ZIRC
ZFIN ID: ZDB-FIG-090223-27
Jin et al., 2009 - Definitive hematopoietic stem/progenitor cells manifest distinct differentiation output in the zebrafish VDA and PBI. Development (Cambridge, England)   136(4):647-654 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 26+ somites to Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. 1 Definitive erythroid cells arise in the PBI but not the VDA. (A-K) Whole-mount in situ hybridization to detect βe1-globin expression in wild-type embryos (A-E) at 22 hpf (A), 2 dpf (B), 3 dpf (C), 4 dpf (D) and 5 dpf (E); runx1w84x mutant (F,G) and morphant (H) embryos at 22 hpf (F) and 4 dpf (G,H); and vltm651 embryos (I-K) at 22 hpf (I), 2 dpf (J) and 4 dpf (K). The blue arrow in E indicates βe1-globin expression in pronephros. The insets are higher magnification (20x) views of the corresponding boxed regions (blue).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
hbae1.1 WT standard conditions 26+ somites blood island erythroid progenitor cell ISH
Long-pec post-vent region erythroid progenitor cell ISH
Protruding-mouth post-vent region erythroid progenitor cell ISH
Day 4 post-vent region erythroid lineage cell ISH
Day 5 post-vent region erythroid lineage cell ISH
Day 5 pronephros ISH
WT + MO2-runx1 + MO3-runx1 standard conditions Day 4 Not Detected post-vent region erythroid lineage cell ISH
gata1am651/m651 standard conditions 26+ somites blood island erythroid progenitor cell ISH
Long-pec post-vent region erythroid progenitor cell ISH
Day 4 post-vent region erythroid progenitor cell ISH
runx1hg1/hg1 standard conditions 26+ somites blood island erythroid progenitor cell ISH
Day 4 Not Detected post-vent region erythroid lineage cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-runx1 + MO3-runx1 standard conditions Day 4 blood island hematopoietic multipotent progenitor cell absent, abnormal
Day 4 definitive hemopoiesis process quality, abnormal
Day 4 erythroid progenitor cell absent, abnormal
Day 4 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell absent, abnormal
runx1hg1/hg1 standard conditions Day 4 blood island hematopoietic multipotent progenitor cell absent, abnormal
Day 4 definitive hemopoiesis process quality, abnormal
Day 4 erythroid progenitor cell absent, abnormal
Day 4 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development