FIGURE

Fig. 1

ID

ZDB-FIG-090223-17

Publication

Hinits et al., 2009 - Differential requirements for myogenic regulatory factors distinguish medial and lateral somitic, cranial and fin muscle fibre populations

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Myf5 or Myod are required for slow fibre formation. Dorsal flatmounts of zebrafish embryos injected with the indicated MOs and uninjected controls (con), analysed at the 13- to 15-somite stage by whole-mount immunohistochemistry for MyHC (red) or in situ mRNA hybridisation for the indicated mRNAs (blue). Anterior to top. (Left panels) myf5+myod MOs ablated all slow myosin heavy chain (smyhc1 and slow MyHC, arrowheads), but did not completely prevent actin or tpma expression (arrowheads). myod MO slightly delayed smyhc mRNA and MyHC accumulation (arrows), whereas other single MOs had little effect. (Right panels) Single MRF MOs had no effect on adaxial expression of the other MRFs. However, the myod MO reduced myog signal in fast muscle precursors (white arrows). The box region is shown magnified beneath. Note the increase in signal for each MRF caused by its cognate MO (asterisks). s, somites.

Expression Data

Genes:	acta1b myf5 myf6 myod1 myog smyhc1 tpma
Fish:	WT WT + MO1-myod1 WT + MO1-myog WT + MO3-myf5 WT + MO1-myod1 + MO3-myf5
Knockdown Reagents:	MO1-myod1 MO1-myog MO3-myf5
Anatomical Terms:	adaxial cell segmental plate somite
Stage Range:	10-13 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development