ZFIN ID: ZDB-FIG-081107-8
Kim et al., 2008 - Frizzled 8a function is required for oligodendrocyte development in the zebrafish spinal cord. Developmental dynamics : an official publication of the American Association of Anatomists   237(11):3324-3331 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 2 fz8a function is not required for dorso-ventral patterning of the spinal cord. A, B: Tg(hsp70:fz8aCRDTM-egfp) embryos were heat shocked at 18 hpf. A: Control embryos had high levels of EGFP-fluorescence in the whole embryos. B: Embryos injected with fz8a MO had low levels of EGFP fluorescence, suggesting that fz8a MO works specifically to knock-down fz8a expression. C-F: Transverse sections of the spinal cord of Tg(olig2:egfp) embryos. C, E: Control embryos that show olig2-EGFP and iro3 expression (C), or olig2-EGFP and nk2.2 expression (E), revealed by in situ RNA hybridization. D, F: Tg(olig2:egfp) embryos injected with fz8a MO and hybridized by iro2 (D) and nk2.2 (F) RNA probes.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu4Tg + MO1-fzd8a(AB) heat shock Prim-5 whole organism IFL
knu4Tg(AB) heat shock Prim-5 whole organism IFL
vu12Tg standard conditions Prim-5 spinal cord IFL
vu12Tg + MO1-fzd8a standard conditions Prim-5 spinal cord IFL
irx3a vu12Tg standard conditions Prim-5 spinal cord ISH
vu12Tg + MO1-fzd8a standard conditions Prim-5 spinal cord ISH
nkx2.2a vu12Tg standard conditions Prim-5 spinal cord ISH
vu12Tg + MO1-fzd8a standard conditions Prim-5 spinal cord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
vu12Tg + MO1-fzd8a standard conditions Prim-5 spinal cord dorsal/ventral patterning occurrence, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.