FIGURE

Fig. 7

ID

ZDB-FIG-080923-8

Publication

Tallafuss et al., 2008 - The Met receptor tyrosine kinase prevents zebrafish primary motoneurons from expressing an incorrect neurotransmitter

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Fig. 7

Met is required for aspects of CaP identity. (a) A met MO-injected mn2Et (green) transgenic embryo labeled with znp1 antibody (red) showing that CaP has both a peripheral axon (arrow) and a central axon (asterisk). (a′) The same cell showing only GFP. (a″) The same cell showing only znp1 labeling. (b,c) Islet1/2 antibody (Isl; red) and zn1 plus znp1 antibodies (green) labeling showing that CaPs in met MO-injected embryos (c) co-express these markers as they do in controls (b). For each condition we examined eight spinal hemisegments per embryo. n = 56 met MO-injected embryos and 32 control embryos. (d) chat is expressed in somata in the normal location of PMNs; gray lines show segment boundaries. (e,f) Expression of chat (green) and the CaP and VaP-specific marker islet2 (red) shows that in met MO-injected embryos (f) CaPs express chat, as they do in controls (e). For each condition we examined eight hemisegments per embryo. n = 24 met MO-injected embryos and 15 control embryos. (g) Co-expression of chat (red) and met (green) in PMNs. Scale bars, 10 μm.

Expression Data

Genes:	chata GFP isl2a met
Antibodies:	zn-1 znp-1
Fish:	parga^mn2Et parga^mn2Et + MO1-met + MO2-met
Knockdown Reagents:	MO1-met MO2-met
Anatomical Terms:	CaP motoneuron CaP motoneuron axon CaP motoneuron neuronal cell body motor neuron primary motor neuron neuronal cell body
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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