ZFIN ID: ZDB-FIG-080904-63
Liu et al., 2008 - Fli1 Acts at the Top of the Transcriptional Network Driving Blood and Endothelial Development. Current biology : CB   18(16):1234-1240 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 5-9 somites to 10-13 somites
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 5-9 somites to 10-13 somites

Fig. S7 Loss- and gain-of function experiments reveal differential control of Fli1 expression by BMP signalling.
Zebrafish embryos transgenic for a heat shock inducible, dominant negative Bmp receptor were heat shocked at 30% epiboly and processed for WISH at 10s (A-F) or 6s (I,J). (G) Expanded WISH signals for Fli1 (red arrows) in dino mutants. (H) No expansion of Nkx2.5 and Krox20 in dino mutants. Dorsal views.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
egr2b AB standard conditions 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
chrdtm84/tm84 standard conditions 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
fli1 AB standard conditions 10-13 somites posterior lateral mesoderm ISH
chrdtm84/tm84 standard conditions 10-13 somites posterior lateral mesoderm ISH
w30Tg control 5-9 somites anterior lateral mesoderm ISH
10-13 somites posterior lateral mesoderm ISH
w30Tg heat shock 5-9 somites anterior lateral mesoderm ISH
10-13 somites Not Detected posterior lateral mesoderm ISH
gata2a w30Tg control 10-13 somites posterior lateral mesoderm ISH
w30Tg heat shock 10-13 somites Not Detected posterior lateral mesoderm ISH
myod1 AB standard conditions 10-13 somites somite ISH
chrdtm84/tm84 standard conditions 10-13 somites somite ISH
nkx2.5 AB standard conditions 10-13 somites heart primordium ISH
chrdtm84/tm84 standard conditions 10-13 somites heart primordium ISH
tal1 w30Tg control 10-13 somites posterior lateral mesoderm ISH
w30Tg heat shock 10-13 somites Not Detected posterior lateral mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
chrdtm84/tm84 standard conditions 10-13 somites embryonic hemopoiesis disrupted, abnormal
10-13 somites posterior lateral mesoderm morphology, abnormal
w30Tg heat shock 5-9 somites anterior lateral mesoderm angioblastic mesenchymal cell morphology, normal
10-13 somites embryonic hemopoiesis disrupted, abnormal
10-13 somites posterior lateral mesoderm angioblastic mesenchymal cell morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.