ZFIN ID: ZDB-FIG-081231-1
Liu et al., 2008 - Fli1 Acts at the Top of the Transcriptional Network Driving Blood and Endothelial Development. Current biology : CB   18(16):1234-1240 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 5-9 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 5-9 somites

Fig. S6 Fli1 expression in the PLM is independent of Cloche at an early stage.
(A,B) Whole mount in situ hybridisation showing Fli1 expression in wild type (A) and Cloche (B) embryos at 6s. Note that PLM expression of Fli1 is retained in Cloche (black arrows), whereas ALM expression is absent (red arrows); (C,D) Erg expression in wild type and Clo embryos; (E,F) Gata2 expression in wild type and Clo embryos; (G,H) Scl expression in wild type and Clo embryos; (I,J) Etsrp expression in wt and Clo embryos. Note that both ALM and PLM expression of Erg (D, red arrows), Scl (H, red arrows) and Etsrp (J, red arrows), and PLM expression of Gata2 (F, red arrows) are absent in Clo embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
erg AB standard conditions 5-9 somites anterior lateral mesoderm ISH
5-9 somites posterior lateral mesoderm ISH
npas4ls5/s5 standard conditions 5-9 somites Not Detected anterior lateral mesoderm ISH
5-9 somites Not Detected posterior lateral mesoderm ISH
etsrp AB standard conditions 5-9 somites anterior lateral mesoderm ISH
5-9 somites posterior lateral mesoderm ISH
npas4ls5/s5 standard conditions 5-9 somites Not Detected anterior lateral mesoderm ISH
5-9 somites Not Detected posterior lateral mesoderm ISH
fli1 AB standard conditions 5-9 somites anterior lateral mesoderm ISH
5-9 somites posterior lateral mesoderm ISH
npas4ls5/s5 standard conditions 5-9 somites Not Detected anterior lateral mesoderm ISH
5-9 somites posterior lateral mesoderm ISH
gata2a AB standard conditions 5-9 somites posterior lateral mesoderm ISH
npas4ls5/s5 standard conditions 5-9 somites Not Detected posterior lateral mesoderm ISH
tal1 AB standard conditions 5-9 somites anterior lateral mesoderm ISH
5-9 somites posterior lateral mesoderm ISH
npas4ls5/s5 standard conditions 5-9 somites Not Detected anterior lateral mesoderm ISH
5-9 somites Not Detected posterior lateral mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
npas4ls5/s5 standard conditions 5-9 somites anterior lateral mesoderm morphology, abnormal
5-9 somites embryonic hemopoiesis disrupted, abnormal
5-9 somites posterior lateral mesoderm morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.