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ZFIN ID: ZDB-FIG-080613-32
Poon et al., 2008 - Expression of zebrafish nos2b surrounds oral cavity. Developmental dynamics : an official publication of the American Association of Anatomists   237(6):1662-1667 Full text @ Dev. Dyn.
Anatomical Terms:
Stage Range: 1-cell to Day 4

Fig. 1 Expression profiling of nos2b in zebrafish embryos by reverse transcription-polymerase chain reaction (RT-PCR) and whole-mount in situ hybridization (WISH). A: Chromosomal mapping of nos2b. Linkage map showing the localization of nos2b (=unp2188) to LG15 (obtained from http://wwwmap.tuebingen.mpg.de/). The distances between markers on the main map (left) are given in centimorgans (cM) and in centirays (cR) for the expanded region (right). B: Detection of nos2b transcripts by RT-PCR revealed a lower maternal expression in contrast to a higher expression after 16 hours postfertilization (hpf). An internal control using the house-keeping gene, elongation factor 1 alpha (ef1α) is also shown. C-E: nos2b transcripts were first detected at 14 hpf in the prospective ventral diencephalon. Expression of nos2b (arrowheads in C,D,E,F and G) is confined to the anterior part of the forebrain as shown in the frontal view (D), lateral view and the flat mount ventral view (E) of the 14 hpf embryo double stained with nos2b and catL1b. The gray dotted line in E outlined the optic primordium (op). F: By 20 hpf nos2b remained in the ventral floor of the forebrain. G: However, nos2b is excluded from the lim3-positive anterior pituitary. H-J: In addition, nos2b expression domain is ventrad to the nk2.1-positive hypothalamus (H,I) and colocalizes with fgf3 (J). d, diencephalon; e, eye; hg, hatching gland; ht, hypothalamus; pit, pituitary; t, telencephalon.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb AB standard conditions 10-13 somites hatching gland ISH
fgf3 AB control Prim-15 diencephalon ISH
lhx3 AB standard conditions 26+ somites adenohypophyseal placode ISH
nkx2.4b AB standard conditions 26+ somites hypothalamus ISH
nos2b AB control Prim-15 diencephalon ISH
AB standard conditions 1-cell whole organism RTPCR
10-13 somites diencephalon ISH
20-25 somites floor plate diencephalic region ISH
26+ somites diencephalon ISH
Prim-5 whole organism RTPCR
Day 4 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.