FIGURE

Fig. 3

ID

ZDB-FIG-080417-31

Publication

Sachidanandan et al., 2008 - Identification of a novel retinoid by small molecule screening with zebrafish embryos

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Fig. 3

DTAB affects the expression of early developmental markers.
In situ hybridizations of zebrafish embryos show that treatment with DTAB results in severe reduction of krox20, pax2a, myoD, ntl, gsc, and raldh2 staining. DTAB treated embryos exhibited an upregulation of cyp26a1 transcripts in the tail and head. Embryos were treated with 12.5 μM DTAB or DMSO control from 4hpf onwards until fixation. Embryos were fixed at 75% epiboly (G?L), 10-somite (M?N), 18-somite (C?F) and 26-somite (A,B) stages. A?B, E?F, M?N are lateral views with anterior to the left and dorsal to the top. C?D are dorsal views with anterior to the left. G?L are dorsal views with the animal pole to the top.

Expression Data

Genes:	aldh1a2 cyp26a1 egr2b gsc myod1 pax2a tbxta
Fish:	AB/TU
Condition:	chemical treatment: dodecyltrimethylammonium bromide
Anatomical Terms:	axial chorda mesoderm brain midbrain hindbrain boundary optic stalk otic vesicle prechordal plate presumptive paraxial mesoderm pronephric duct rhombomere 3 rhombomere 5 segmental plate somite spinal cord neural tube tail bud
Stage Range:	75%-epiboly to 26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU
Condition:	chemical treatment: dodecyltrimethylammonium bromide
Observed In:	anterior/posterior pattern specification
Stage Range:	75%-epiboly to 26+ somites

Phenotype Detail

Acknowledgments

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Open Access

Full text @ PLoS One