FIGURE

Fig. 7

ID

ZDB-FIG-080416-13

Publication

Huang et al., 2008 - Histamine metabolism influences blood vessel branching in zebrafish reg6 mutants

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Fig. 7

Blocking of histamine synthesis causes blood vessel branching defects in wild-type zebrafish embryos. (A) In a 32 hpf wild-type embryo, the developing caudal vein formed a transient vascular plexus. (B) When treated with 900 μM of the histamine synthesis blocker, urocanic acid, the caudal vein of a wild-type sibling formed much fewer vascular branches and even developed a reg6-like swollen lumen. Red arrows, caudal veins. (C) Quantitative results of the suppression of urocanic acid on blood vessel branching in wild-type zebrafish embryos. Two clutches of wild-type embryos (blue and red bars) were treated with the vehicle (0.0015 hydrochloric acid), or 300 or 900 μM of urocanic acid in egg water, from 26–36 hpf at 28.5°C. The percentage of embryos with less branched caudal vein was scored at the end of treatment (Y-axis, n = 40 unless otherwise specified).

Expression Data

Genes:	EGFP egr1
Fish:	y1Tg reg6^j21/j21; y1Tg
Conditions:	chemical treatment: pharmaceutical physical alteration: anatomical structure
Anatomical Terms:	caudal vein plexus unspecified
Stage Range:	Prim-15 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg
Condition:	chemical treatment: pharmaceutical
Observed In:	caudal vein plexus
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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