FIGURE

Fig. 5

ID

ZDB-FIG-080416-11

Publication

Huang et al., 2008 - Histamine metabolism influences blood vessel branching in zebrafish reg6 mutants

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Fig. 5

Suppression of the reg6 regenerating vessel phenotype by SKF91488 and histamine. (A) reg6 regenerating vessels formed swollen lumina 3 days post amputation (dpa) at 33°C (yellow arrows). (B) In the presence of 10 μM SKF91488, reg6 mutants developed significantly smaller and fewer swollen vessels (yellow arrows). Red arrows, amputation plane. (C) Quantitative data of the suppression of reg6 regenerating vessels by SKF91488 (n = 5 for each group). This experiment was repeated at least 3 times, and consistent results were observed. (D) Quantitative polymerase chain reaction (QPCR) analyses showed elevated levels of histamine methyltransferase (HMT) RNA in both reg6 2 and 3-dpa regenerates compared with the wild-type. (E) Both SKF91488 and histamine could reverse the reg6 vessel phenotypes. The number of swollen vessels in reg6 mutants was counted at 3 dpa at 33°C (blue bars; B, before treatment) before they were transferred to either fresh water (H₂O), 10 μM SKF91488, or 1 mM histamine. The number of swollen vessels was counted again 12 h later (red bars; P, post-treatment). Shown is a representative set of data with 10 fish. Similar results were obtained from 2 independent experiments with different stocks of reg6 fish.

Expression Data

Gene:	EGFP
Fish:	reg6^j21/j21; y1Tg
Conditions:	physical alteration: anatomical structure chemical treatment: EC 2.1.1.8 (histamine N-methyltransferase) inhibitor, physical alteration: anatomical structure
Anatomical Terms:	fin vasculature regenerating fin
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	reg6^j21/j21; y1Tg
Condition:	chemical treatment: EC 2.1.1.8 (histamine N-methyltransferase) inhibitor
Observed In:	fin vasculature
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.