FIGURE

Fig. 5

ID

ZDB-FIG-080411-28

Publication

Pagnon-Minot et al., 2008 - Collagen XV, a novel factor in zebrafish notochord differentiation and muscle development

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Fig. 5

The lack of collagen XV affects the nototochord differentiation. (A) In 24 hpf wild type embryos, the notochord visualized by Nomarsky microscopy shows cells with large vacuoles. (B) In collagen XV morphants, these vacuoles are smaller. Whole mount in situ hybridization of 24 hpf (C, E and inserts in G, H) and 30 hpf (G, H) wild-type (C, E, G) and col15a1-MO (D, F, H) zebrafish embryos with Ehh (C, D) and Col2a1 (E–H) probes. In Col15a1-MO, a persistent strong expression of the early chordamesoderm marker, ehh (D) and of Col2a1 (F, H) is observed in notochord. At 24 hpf, ehh expression (C) disappears and Col2a1 expression (E) is restricted to the floor plate (arrow) and the hypochord (arrowhead) n wild type embryos. (I, J) Laminin immunostaining in wild-type (I) and col15a1-MO (J) embryos. In morphants, the basement membrane is more diffuse and exhibits several staining interruptions. In figures A–F and I, J, the embryo head is on the left and the dorsal side on the top. Magnification: A, B: x 600; C–F: x 300; G, H: x 900; I, J: x 400.

Expression Data

Genes:	col2a1a ihhb
Fish:	WT WT + MO1-col15a1a
Knockdown Reagent:	MO1-col15a1a
Anatomical Terms:	floor plate hypochord notochord
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-col15a1a
Knockdown Reagent:	MO1-col15a1a
Observed In:	notochord basement membrane notochord vacuole
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 316(1), Pagnon-Minot, A., Malbouyres, M., Haftek-Terreau, Z., Kim, H.R., Sasaki, T., Thisse, C., Thisse, B., Ingham, P.W., Ruggiero, F., and Le Guellec, D., Collagen XV, a novel factor in zebrafish notochord differentiation and muscle development, 21-35, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.