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ZFIN ID: ZDB-FIG-080411-31
Pagnon-Minot et al., 2008 - Collagen XV, a novel factor in zebrafish notochord differentiation and muscle development. Developmental Biology   316(1):21-35 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 8 The lack of collagen XV disrupts slow and fast muscle patterning. (A, B) Lateral views of the myotomal segments of 48 hpf wild-type (A) and Col15a1-MO (B) embryos, revealing the slow and fast fibers stained with phalloïdin-FITC (arrows). (B) Similar views of morphants reveal an important disorganization of myofibrils. (C) 48 hpf wild-type and (D) Col15a1-MO smhc-GFP transgenic embryos, showing the organization of the slow fibers. Note that the slow fibers are collapsed in the Col15a1-MO embryos and that the nuclei are less visible. (E, F) Medial views of wild-type (E) and Col15a1-MO (F) embryos, revealing the fast fibers stained with mAb F310. At 48 hpf, only few Col15a1-MO fast fibers are observed, and large areas of the muscle are not stained (arrows). (G, H) Lateral views of 48 hpf wild-type (G) and Col15a1-MO (H) staining with β-catenin antibodies show that the lack of collagen XV does not lead to a decrease in muscle cell number. Magnification: A–H: x 300.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP i108Tg control Long-pec muscle myofibril IFL
i108Tg + MO1-col15a1a standard conditions Long-pec muscle myofibril IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F310 IHC WT control Long-pec fast muscle cell contractile fiber
IHC WT + MO1-col15a1a standard conditions Long-pec fast muscle cell contractile fiber
Ab1-ctnnb IHC WT control Long-pec muscle cell
IHC WT + MO1-col15a1a standard conditions Long-pec muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-col15a1a standard conditions Long-pec fast muscle cell myofibril disorganized, abnormal
Long-pec fast muscle cell myofibril misaligned with fast muscle cell myofibril, abnormal
Long-pec slow muscle cell myofibril disorganized, abnormal
Long-pec slow muscle cell myofibril misaligned with slow muscle cell myofibril, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 316(1), Pagnon-Minot, A., Malbouyres, M., Haftek-Terreau, Z., Kim, H.R., Sasaki, T., Thisse, C., Thisse, B., Ingham, P.W., Ruggiero, F., and Le Guellec, D., Collagen XV, a novel factor in zebrafish notochord differentiation and muscle development, 21-35, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.