|ZFIN ID: ZDB-FIG-080411-31|
Fig. 8 The lack of collagen XV disrupts slow and fast muscle patterning. (A, B) Lateral views of the myotomal segments of 48 hpf wild-type (A) and Col15a1-MO (B) embryos, revealing the slow and fast fibers stained with phalloïdin-FITC (arrows). (B) Similar views of morphants reveal an important disorganization of myofibrils. (C) 48 hpf wild-type and (D) Col15a1-MO smhc-GFP transgenic embryos, showing the organization of the slow fibers. Note that the slow fibers are collapsed in the Col15a1-MO embryos and that the nuclei are less visible. (E, F) Medial views of wild-type (E) and Col15a1-MO (F) embryos, revealing the fast fibers stained with mAb F310. At 48 hpf, only few Col15a1-MO fast fibers are observed, and large areas of the muscle are not stained (arrows). (G, H) Lateral views of 48 hpf wild-type (G) and Col15a1-MO (H) staining with β-catenin antibodies show that the lack of collagen XV does not lead to a decrease in muscle cell number. Magnification: A–H: x 300.
|i108Tg + MO1-col15a1a||standard conditions||Long-pec||IFL|
|IHC||WT + MO1-col15a1a||standard conditions||Long-pec|
|IHC||WT + MO1-col15a1a||standard conditions||Long-pec||muscle cell|
|WT + MO1-col15a1a||standard conditions||Long-pec||fast muscle cell myofibril disorganized, abnormal|
|Long-pec||fast muscle cell myofibril misaligned with fast muscle cell myofibril, abnormal|
|Long-pec||slow muscle cell myofibril disorganized, abnormal|
|Long-pec||slow muscle cell myofibril misaligned with slow muscle cell myofibril, abnormal|
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Reprinted from Developmental Biology, 316(1), Pagnon-Minot, A., Malbouyres, M., Haftek-Terreau, Z., Kim, H.R., Sasaki, T., Thisse, C., Thisse, B., Ingham, P.W., Ruggiero, F., and Le Guellec, D., Collagen XV, a novel factor in zebrafish notochord differentiation and muscle development, 21-35, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.