ZFIN Image: Pagnon-Minot et al., 2008, Fig. 5

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Fig. 5 The lack of collagen XV affects the nototochord differentiation. (A) In 24 hpf wild type embryos, the notochord visualized by Nomarsky microscopy shows cells with large vacuoles. (B) In collagen XV morphants, these vacuoles are smaller. Whole mount in situ hybridization of 24 hpf (C, E and inserts in G, H) and 30 hpf (G, H) wild-type (C, E, G) and col15a1-MO (D, F, H) zebrafish embryos with Ehh (C, D) and Col2a1 (E–H) probes. In Col15a1-MO, a persistent strong expression of the early chordamesoderm marker, ehh (D) and of Col2a1 (F, H) is observed in notochord. At 24 hpf, ehh expression (C) disappears and Col2a1 expression (E) is restricted to the floor plate (arrow) and the hypochord (arrowhead) n wild type embryos. (I, J) Laminin immunostaining in wild-type (I) and col15a1-MO (J) embryos. In morphants, the basement membrane is more diffuse and exhibits several staining interruptions. In figures A–F and I, J, the embryo head is on the left and the dorsal side on the top. Magnification: A, B: x 600; C–F: x 300; G, H: x 900; I, J: x 400.

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Reprinted from Developmental Biology, 316(1), Pagnon-Minot, A., Malbouyres, M., Haftek-Terreau, Z., Kim, H.R., Sasaki, T., Thisse, C., Thisse, B., Ingham, P.W., Ruggiero, F., and Le Guellec, D., Collagen XV, a novel factor in zebrafish notochord differentiation and muscle development, 21-35, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.