ZFIN ID: ZDB-FIG-080328-10
Liedtke et al., 2008 - Midkine-b regulates cell specification at the neural plate border in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   237(1):62-74 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 1-4 somites to 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 1-4 somites to Prim-5

Fig. 6 mdkb regulates formation of sensory neurons. A-H: Dorsal views of trunk regions in noninjected (A,C,E,G) and mdkb Morpholino (MO) -injected embryos (B,D,F,H) at the one- to three-somite stage showing ngn1 (A-F) and isl1 (G,H) expression. Note that the embryo in H is slightly delayed due to MO injection. Dorsal proneural cells (dpnc) are reduced in injected embryos at mid-hindbrain (arrows in A,B) and trunk level (arrows in C-H), while number of ventral proneural cells (vpnc) is not affected. Note remaining interneurons (arrowheads), but absent sensory neurons (arrows) in mdkb morphants (in E,F). I-K: Lateral views of trunk regions in embryos at the 18-somite stage showing isl2 expression in ventrally located motor neurons (arrowheads) and dorsal sensory Rohon-Beard (RB) neurons (arrows). Embryos injected with 150 pg of mdkb RNA (J) show enhanced numbers of dorsal RB, while motor neurons are not affected. In contrast, RB are reduced in mdkb MO-injected embryos (K). L-Q: Dorsal views of embryos at 28 hours postfertilization (hpf) immunostained with an antibody against acetylated tubulin. L-N: Focal planes at the level of dorsal sensory neurons. O-Q: Focal planes of the same embryos at the level of ventral motor neurons. Note increased RB cell number in mdkb RNA injected embryos (arrow in M) and reduced RB cell number in mdkb MO-injected embryos (arrow in N), while number of motor neurons is not affected (arrowheads in M-O). DHb, dorsal hindbrain; TG, trigeminal ganglia; VMb, ventral midbrain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
isl1a AB standard conditions 1-4 somites proneural cluster ISH
AB + MO1-mdkb + MO2-mdkb standard conditions 1-4 somites proneural cluster ISH
isl2a AB standard conditions 14-19 somites motor neuron ISH
14-19 somites Rohon-Beard neuron ISH
AB + MO1-mdkb + MO2-mdkb standard conditions 14-19 somites motor neuron ISH
14-19 somites Rohon-Beard neuron ISH
neurog1 AB standard conditions 1-4 somites cranial ganglion ISH
1-4 somites hindbrain neural plate ISH
1-4 somites presumptive diencephalon ISH
1-4 somites proneural cluster ISH
AB + MO1-mdkb + MO2-mdkb standard conditions 1-4 somites cranial ganglion ISH
1-4 somites hindbrain neural plate ISH
1-4 somites presumptive diencephalon ISH
1-4 somites proneural cluster ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-mdkb + MO2-mdkb standard conditions 1-4 somites proneural cluster decreased size, abnormal
1-4 somites sensory neuron decreased amount, abnormal
14-19 somites motor neuron amount, normal
14-19 somites Rohon-Beard neuron decreased amount, abnormal
Prim-5 motor neuron amount, normal
Prim-5 Rohon-Beard neuron decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.