FIGURE

Fig. 4

ID

ZDB-FIG-080328-8

Publication

Liedtke et al., 2008 - Midkine-b regulates cell specification at the neural plate border in zebrafish

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Fig. 4

mdkb regulates early pax3 expression. A: Lateral view of pax3 expression in a control embryo at 80% epiboly (dorsal to the right). B: Reduced pax3 expression in embryo co-injected with mdkb Up and Down splice-site Morpholinos (MOs). The arrow marks an area with reduced pax3 expression. C: Expanded pax3 expression in an embryo injected with 150 pg of mdkb mRNA. The arrow marks a region of expanded pax3 expression, the arrowhead the anterior border of pax3 expression. D,E: otx2 expression in the anterior neural plate of control (D) and mdkb MO-injected embryos (E) at 90% epiboly. Note that the otx2 region is slightly expanded, suggesting that neural plate is not reduced in mdkb morphants.

Expression Data

Genes:	otx2b pax3a
Fish:	AB AB + MO1-mdkb + MO2-mdkb
Knockdown Reagents:	MO1-mdkb MO2-mdkb
Anatomical Terms:	anterior neural plate neural plate
Stage Range:	75%-epiboly to 90%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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