ZFIN ID: ZDB-FIG-080325-76
Bessa et al., 2008 - meis1 regulates cyclin D1 and c-myc expression, and controls the proliferation of the multipotent cells in the early developing zebrafish eye. Development (Cambridge, England)   135(5):799-803 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: 20-25 somites

Fig. 3 meis1 is required for the G1-S transition and the expression of the G1-S regulators cyclin D1 and c-myc. (A-D) Histograms displaying DNA content (cell cycle profile) of cells from dissected 19hpf eyes of meis1-MO treated embryos relative to (A) controls (Crtl-MO), (B) meis1-MO+GFP-meis1 mRNA, (C) meis1-MO+cyclin D1 mRNA, and (D) meis1-MO+c-myc mRNA injected embryos. Number of replicates (n) and P values are indicated. meis1 knockdown induces a delay in G1, which is partially rescued by co-injection of GFP-meis1 (B), cyclin D1 (C) and c-myc (D) mRNAs. (E) Average percentages are shown for G1, S and G2/M DNA content. The cell-cycle profiles of control morphants and of uninjected, wild-type embryos are indistinguishable (not shown). (F-I) In situ analysis of cyclin D1 and c-myc transcription in control (F,H) and meis1 morphants (G,I) at 19hpf. meis1 morphants show a dramatic reduction in cyclin D1 and c-myc in the eye (red arrowheads). cyclin D1 and c-myc are still detected in other body regions (black arrowheads). Hybridization and reaction development were performed strictly in parallel. Representative embryos are shown.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccnd1 WT control 20-25 somites optic vesicle ISH
WT + MO1-meis1b standard conditions 20-25 somites optic vesicle ISH
myca WT control 20-25 somites optic vesicle ISH
WT + MO1-meis1b standard conditions 20-25 somites optic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development