FIGURE

Fig. 4

ID

ZDB-FIG-080211-5

Publication

Aquilina-Beck et al., 2007 - Nodal signaling is required for closure of the anterior neural tube in zebrafish

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Fig. 4

The expanded and divided pineal phenotype is present in n-cad mutants and morphants. (A-C) Homozygous pac mutants were fixed at 30 hpf, and then processed for in situ hybridization with an antisense probe for otx5. Dorsal views with anterior to the top. pac mutants could have a (A) normal, round-shaped pineal morphology, (B) an elongated pineal anlage or (C) a divided pineal. (D-G) Embryos were injected at the one to four cell stage, fixed at 74 hpf, and then assayed for expression of otx5. Frontal views with dorsal to the top. The presumptive pineal organ (arrowheads) forms (D) a single domain in control embryos and a (E-G) divided pineal in embryos depleted in N-cad through morpholino (MO) injections. Scale bars: 30 μm (A-C), 100 μm (D-G).

Expression Data

Gene:	otx5
Fish:	WT cdh2^{p79emcf/p79emcf} WT + MO1-cdh2
Knockdown Reagent:	MO1-cdh2
Anatomical Term:	epiphysis
Stage Range:	Prim-15 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdh2^{p79emcf/p79emcf} WT + MO1-cdh2
Knockdown Reagent:	MO1-cdh2
Observed In:	epiphysis
Stage Range:	Prim-15 to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.