N-cad expression in the anterior neural keel is altered in MZoep mutants. (A-F) N-cad immunostaining of tissue sections from WT and MZoep embryos. (A, B) Sagittal sections though the anterior neural keel with anterior to the left and dorsal to the top. (C-D') Parasagittal sections with anterior to the left and dorsal to the top. Panels C' and D' are higher magnifications of the boxed regions in panels C and D, respectively. Arrows point to N-cad labeling in the cell membrane, while the arrowhead indicates labeling in the cytoplasm. (E, F) Transverse sections through the anterior neural tube, dorsal to the top. n e 30 embryos for each sample. Embryos are at 95?100% epiboly (95?100%) or the 5?6 somite stage (5?6 s). Scale bars: 40 μm (C, D, E, F), 20 μm (A, B, C', D').
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