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ZFIN ID: ZDB-FIG-080211-2
Aquilina-Beck et al., 2007 - Nodal signaling is required for closure of the anterior neural tube in zebrafish. BMC Developmental Biology   7(1):126 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-4 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to Prim-5

Fig. 1 Pineal precursors fail to reach the midline of the forebrain in squint mutants. (A-C) WT embryos were processed for whole mount in situ hybridization with an antisense probe for the gene flh, which is expressed in the pineal precursors (arrowheads). Images are dorsal views of the entire embryo, anterior to the top. (A) At the 2–3 somite stage, pineal precursors are located in two widely spaced lateral domains. (B) By the 5–6 somite stage, these domains have moved towards the dorsal midline of the forebrain. (C) By the 7–8 somite stage, a single, round-shaped pineal anlage has formed. (D-F) At 2 dpf, sqt mutant embryos from the same clutch have a wide range of eye phenotypes. Frontal views of live embryos with dorsal to the top. (D) The eyes of WT embryos and some sqt mutants (not shown) are completely separated from one another. (E) Other sqt mutants have partially fused eyes that form two lenses or (F) a single eye with one lens. (G-L) Embryos at the (G-I) 7–8 somite stage and (J-L) 24 hpf were processed for whole mount in situ hybridization with a probe for the pineal gene (G-I) flh or (J-O) otx5, dorsal views, anterior to the top. (G,J) In WT siblings, the pineal precursors (arrowhead) have converged to form a round pineal anlage. In sqt mutants the pineal precursors (arrowheads) form a domain that is (H,K) elongated or (I,L) divided in two. The pineal anlagen of the (M) cyc mutant and the (N) Zoep mutant have a round shape that is similar to that of WT fish, while the pineal precursors of the (O) MZoep mutant are divided in two domains. All images are dorsal views with anterior to the top. Scale bars: 100 μm (A-C, G-I), 70 μm (D-F), 30 μm (J-O).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
noto WT standard conditions 1-4 somites anterior neural keel ISH
5-9 somites presumptive diencephalon ISH
ndr1cz35/cz35 standard conditions 5-9 somites presumptive diencephalon ISH
otx5 WT standard conditions Prim-5 epiphysis ISH
ndr1cz35/cz35 standard conditions Prim-5 epiphysis ISH
ndr2m294/m294 standard conditions Prim-5 epiphysis ISH
tdgf1m134/m134 standard conditions Prim-5 epiphysis ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ndr1cz35/cz35 standard conditions 5-9 somites epiphysis split, abnormal
Prim-5 eye fused with eye, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.