Fig. 9

KBP is required for ENS axonal development, but not for head patterning. (A,B) Lateral views of larvae at 10 dpf showing neurons labeled with anti HuC/D in the posterior hindgut of the enteric nervous system in a WT (A) and kbp^st23 mutant (B). There were no discernable differences in neuronal organization between kbp^st23 mutants and siblings. (C,D) TEM images of transverse sections through the enteric nervous system at 7 dpf shows a group of small axons with organized microtubules (arrowheads) in wild type (C) and more disorganized axons with accumulations of vesicles (arrow) in the mutant (D). Scale bars: 200 nm. (E,F) Ventral views of larvae at 10 dpf showing head cartilages labeled by Alcian Blue in a wild type (E) and kbp^st23 mutant (F). There were no discernable differences in the organization of the head skeleton between kbp^st23 mutants and siblings.