ZFIN ID: ZDB-FIG-070927-39
Yu et al., 2005 - Semaphorin signaling guides cranial neural crest cell migration in zebrafish. Developmental Biology   280(2):373-385 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Fig. 4 Zebrafish nrps interact with lzr/pbx4 during cranial NCC migration. nrp expression is shown in blue in A–H; krox20 and dlx2 are in pink or orange in A–H and blue in I–L. Arrows indicate the second cranial NC stream. (A–B) nrp1a is expressed on the first cranial NC stream (arrowhead). (C–D) nrp1b is expressed in a subset of proximal cranial NCCs contributing to the cranial sensory ganglia (arrowheads). (E–H) nrp2a in E-F and nrp2b in G–H are expressed in cranial NCCs. nrp2a is expressed strongly on the first and third cranial NC streams and weakly on the second cranial NC stream, while nrp2b is expressed in all three streams. Arrowheads indicate the third cranial NC stream in E and G. In lzr/pbx4 mutants, nrp expression in the cranial NC is disorganized in a manner corresponding to the disorganization of cranial NCCs. (I–L) MO knockdown of Nrp2a (K) or Nrp2b (L) results in the restoration of the normal cranial NCC migratory pattern. Asterisks mark the cranial NC-free zone in I. K and L and cranial NCCs in this normally cranial NC-free zone in lzr/pbx4 mutants (J). Scale bar: 40 μm in A–L.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx2a WT standard conditions 14-19 somites cranial neural crest cell ISH
pbx4b557/b557 standard conditions 14-19 somites cranial neural crest cell ISH
pbx4b557/b557 + MO1-nrp2a standard conditions 14-19 somites cranial neural crest cell ISH
pbx4b557/b557 + MO1-nrp2b standard conditions 14-19 somites cranial neural crest cell ISH
nrp1a WT standard conditions 14-19 somites cranial neural crest cell ISH
pbx4b557/b557 standard conditions 14-19 somites cranial neural crest cell ISH
nrp1b WT standard conditions 14-19 somites cranial neural crest cell ISH
pbx4b557/b557 standard conditions 14-19 somites cranial neural crest cell ISH
nrp2a WT standard conditions 14-19 somites cranial neural crest cell ISH
pbx4b557/b557 standard conditions 14-19 somites cranial neural crest cell ISH
nrp2b WT standard conditions 14-19 somites cranial neural crest cell ISH
pbx4b557/b557 standard conditions 14-19 somites cranial neural crest cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pbx4b557/b557 standard conditions 14-19 somites cranial neural crest cell mislocalised, abnormal
14-19 somites neural crest cell migration disrupted, abnormal
pbx4b557/b557 + MO1-nrp2a standard conditions 14-19 somites neural crest cell migration occurrence, normal
pbx4b557/b557 + MO1-nrp2b standard conditions 14-19 somites neural crest cell migration occurrence, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 280(2), Yu, H.H., and Moens, C.B., Semaphorin signaling guides cranial neural crest cell migration in zebrafish, 373-385, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.