ZFIN ID: ZDB-FIG-070911-7
Perner et al., 2007 - The Wilms tumor genes wt1a and wt1b control different steps during formation of the zebrafish pronephros. Developmental Biology   309(1):87-96 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 3 Knockdown of wt1a and wt1b leads to different defects during pronephros development. (A) Confocal images of control and wt1 morpholino-injected wt1b::GFP embryos were recorded 24 and 48 h after fertilization and assembled into 3D projections. (B) Formation of kidney cysts (asterisks) in wt1b morpholino-injected embryos 48 h after fertilization was visualized by H&E staining on cross sections. Note that a part of the glomerular filtration unit was still detectable between the cystic structures. nc, notochord; gl, glomerulus; pt, pronephric tubule; pd, pronephric duct.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
li1Tg + MO1-wt1b + MO2-wt1a(AB/TU) standard conditions Prim-5 glomerulus development disrupted, abnormal
Prim-5 pronephric duct malformed, abnormal
Prim-5 pronephric glomerulus aplastic, abnormal
Prim-5 pronephric tubule aplastic, abnormal
Prim-5 pronephros development disrupted, abnormal
Long-pec glomerulus development disrupted, abnormal
Long-pec pronephric duct malformed, abnormal
Long-pec pronephric glomerulus aplastic, abnormal
Long-pec pronephric tubule aplastic, abnormal
Long-pec pronephros development disrupted, abnormal
li1Tg + MO1-wt1b(AB/TU) standard conditions Prim-5 pronephros morphology, normal
Long-pec glomerulus development disrupted, abnormal
Long-pec pronephric tubule cystic, abnormal
Long-pec pronephros development disrupted, abnormal
li1Tg + MO2-wt1a(AB/TU) standard conditions Prim-5 glomerulus development disrupted, abnormal
Long-pec glomerulus development disrupted, abnormal
Long-pec pronephric glomerulus aplastic, abnormal
Long-pec pronephric tubule aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 309(1), Perner, B., Englert, C., and Bollig, F., The Wilms tumor genes wt1a and wt1b control different steps during formation of the zebrafish pronephros, 87-96, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.