ZFIN Morpholino: MO2-wt1a

Target Location

Genome Build: GRCz11Chromosome: 25

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO2-wt1a

Expressed Gene	Anatomy	Figures
mafba	(not) pronephric glomerulus	Fig. 5 from Dong et al., 2015
magi2a	(not) pronephric glomerulus	Fig. 5 from Dong et al., 2015
nphs1	(not) podocyte (not) pronephric glomerulus (not) whole organism	Fig. 4 from Schnerwitzki et al., 2014 Fig. 4 from Perner et al., 2007
nphs2	(not) pronephric glomerulus (not) whole organism	Fig. 5 from Dong et al., 2015 Fig. 4 from Perner et al., 2007

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Phenotype

Phenotype resulting from MO2-wt1a

Phenotype	Fish	Figures
choroid plexus morphology, abnormal	li1Tg + MO2-wt1a (AB/TU)	FIGURE 4 from Hopfenmüller et al., 2022
choroid plexus ab1-cldn5 labeling spatial pattern, abnormal	li1Tg + MO2-wt1a (AB/TU)	FIGURE 4 from Hopfenmüller et al., 2022
choroid plexus structure, abnormal	li1Tg + MO2-wt1a (AB/TU)	FIGURE 4 from Hopfenmüller et al., 2022
glomerular filtration disrupted, abnormal	EKW + MO2-wt1a	Fig. 3 from Hall et al., 2015
glomerulus development disrupted, abnormal	li1Tg + MO2-wt1a	Fig. 4 from Schnerwitzki et al., 2014 Fig. 3 from Perner et al., 2007

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Phenotype of all Fish created by or utilizing MO2-wt1a

Phenotype	Fish	Conditions	Figures
pericardium edematous, abnormal	AB/TU + MO2-wt1a	standard conditions	Fig. 1 from Perner et al., 2007
yolk edematous, abnormal	AB/TU + MO2-wt1a	standard conditions	Fig. 1 , Fig. S2 from Perner et al., 2007
whole organism edematous, abnormal	AB/TU + MO2-wt1a	standard conditions	Fig. 1 from Perner et al., 2007
yolk syncytial layer edematous, abnormal	EKW + MO2-wt1a	standard conditions	Fig. 3 from Hall et al., 2015
podocyte podocyte foot morphology, abnormal	EKW + MO2-wt1a	standard conditions	Fig. 4 from Hall et al., 2015

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Citations

Include unpublished citations

Pollitt, E.J.G., Sánchez-Posada, J., Snashall, C.M., Derrick, C.J., Noël, E.S. (2024) Llgl1 mediates timely epicardial emergence and establishment of an apical laminin sheath around the trabeculating cardiac ventricle. Development (Cambridge, England). 151(13):
Hopfenmüller, V.L., Perner, B., Reuter, H., Bates, T.J.D., Große, A., Englert, C. (2022) The Wilms Tumor Gene wt1a Contributes to Blood-Cerebrospinal Fluid Barrier Function in Zebrafish. Frontiers in cell and developmental biology. 9:809962
Perner, B., Schnerwitzki, D., Graf, M., Englert, C. (2016) Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning. Journal of visualized experiments : JoVE. (110):e53921
Dong, L., Pietsch, S., Tan, Z., Perner, B., Sierig, R., Kruspe, D., Groth, M., Witzgall, R., Gröne, H., Platzer, M., Englert, C. (2015) Integration of Cistromic and Transcriptomic Analyses Identifies Nphs2, Mafb, and Magi2 as Wilms' Tumor 1 Target Genes in Podocyte Differentiation and Maintenance. Journal of the American Society of Nephrology : JASN. 26(9):2118-28
Hall, G., Gbadegesin, R.A., Lavin, P., Wu, G., Liu, Y., Oh, E.C., Wang, L., Spurney, R.F., Eckel, J., Lindsey, T., Homstad, A., Malone, A.F., Phelan, P.J., Shaw, A., Howell, D.N., Conlon, P.J., Katsanis, N., Winn, M.P. (2015) A Novel Missense Mutation of Wilms' Tumor 1 Causes Autosomal Dominant FSGS. Journal of the American Society of Nephrology : JASN. 26(4):831-43
Schnerwitzki, D., Perner, B., Hoppe, B., Pietsch, S., Mehringer, R., Hänel, F., Englert, C. (2014) Alternative splicing of Wilms tumor suppressor 1 (Wt1) exon 4 results in protein isoforms with different functions. Developmental Biology. 393(1):24-32
Perner, B., Englert, C., and Bollig, F. (2007) The Wilms tumor genes wt1a and wt1b control different steps during formation of the zebrafish pronephros. Developmental Biology. 309(1):87-96

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