FIGURE

Fig. 6

ID
ZDB-FIG-070418-29
Publication
von der Hardt et al., 2007 - The Bmp Gradient of the Zebrafish Gastrula Guides Migrating Lateral Cells by Regulating Cell-Cell Adhesion
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Fig. 6

Defects in Convergence, but Not in Dorsoventral Patterning, Are Synergistically Enhanced upon Combined Loss of Chordin and N-Cadherin and in bmp2b Heterozygotes after Moderate n-cadherin Overexpression. (A–D) Lateral overviews of live embryos of indicated genotype at 24 hpf (cdh2 allele pacfr7; chd allele dintt250) (E, F, I, J, M, N) Live embryos with fluorescently labeled mesoderm cells at the onset and end of gastrulation. Coinjection of small amounts of cdh2-MO and chd-MO in wild-type embryos (J) or injection of small amounts of cdh2-mRNA in bmp2b+/- embryos (N) lead to a synergistic enhancement of the defects in convergence movements. (G, H, K, L) Same embryos as shown in (E), (F), (I), (J), at 5-somite stage and after in situ hybridizations for six3b (left, animal views), a marker for anterior neuroectoderm, and szl (right, vegetal views), a marker for posterior mesoderm. The chd/cdh2 double morphant (L) displays a six3b expression domain not smaller than that of the chd single morphant (K), and a szl domain not larger than that of the chd morphant, indicating that the ventralization caused by loss of Chd is not further enhanced by the simultaneous loss of Cdh2.

Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 5-9 somites

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: 50%-epiboly

Phenotype Detail
Acknowledgments
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