ZFIN ID: ZDB-FIG-061226-30
Montero-Balaguer et al., 2006 - The mother superior mutation ablates foxd3 activity in neural crest progenitor cells and depletes neural crest derivatives in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   235(12):3199-3212 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-25 to Day 4

Fig. 5 Neural derivatives of NC are affected by the mosm188 mutation. A,B: In wild types (A) at 24 hpf, sox10 is expressed in late specified pigment and neural precursors migrating ventrally from the dorsal aspect of the neural tube, in the otic vesicle (ov), and in the preotic and postotic clusters of glial precursors (arrowheads). In the mosm188 mutant embryos (B), the otic vesicle expression is unchanged, while expression in all other domains is greatly reduced. C,D: Anti-Hu staining in the trunk of 4-dpf embryos. The wild-type pattern of the segmentally organized DRGs (arrows), enteric neurons (ENS), and sympathetic neurons (sn; arrowheads) is shown in C. In the mosm188 mutant (D), there are only a few scattered DRGs (arrows) and sympathetic neurons (arrowheads). An asterisk marks the area devoid of enteric neurons in the digestive tube. E,F: The reduction of the sympathetic neuron precursors in mosm188 mutants is already evident at 50-hpf embryos stained with the dopamine beta hydroxylase (dbh) riboprobe. G,H: The enteric neuron precursors migrate into the gut tube at 36 hpf as marked by ret-positive cells in wild-type embryos (G). The ret expression is greatly reduced in mosm188 mutants (H). DRG, dorsal root ganglia; ENS, enteric nervous system; ov, otic vesicle; sn, sympathetic neurons.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dbh WT standard conditions Long-pec peripheral nervous system ISH
foxd3m188(AB) standard conditions Long-pec peripheral nervous system ISH
ret WT standard conditions Prim-25 presumptive enteric nervous system ISH
foxd3m188(AB) standard conditions Prim-25 presumptive enteric nervous system ISH
sox10 WT standard conditions Prim-5 neural crest ISH
foxd3m188(AB) standard conditions Prim-5 neural crest ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
foxd3m188(AB) standard conditions Prim-25 enteric nervous system neuron decreased amount, abnormal
Long-pec peripheral nervous system neuron decreased amount, abnormal
Day 4 dorsal root ganglion decreased amount, abnormal
Day 4 enteric nervous system neuron decreased amount, abnormal
Day 4 peripheral nervous system neuron decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.