FIGURE

Fig. 3

ID

ZDB-FIG-061226-17

Publication

Shimizu et al., 2006 - Cdx-Hox code controls competence for responding to Fgfs and retinoic acid in zebrafish neural tissue

Other Figures

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Fig. 3

Ectopic formation of hindbrain neurons in embryos lacking Cdx1a and Cdx4. (A-H) Detection of hindbrain commissure neurons by immunostaining with monoclonal antibody zn-5 of wild-type control (A-D) and cdx1a/4 morphant embryos (E-H) at 3 days post fertilization (dpf). (B,E) Bright field images. Lateral views (A,B,E,F) and high-magnification dorsal views of hindbrain (C,G) and tail regions (D,H) with anterior to the left. zn-5-positive commissure neurons can be recognized by their axonal structures in the hindbrain regions (arrowheads) and ectopically in the posteriormost neural tissue (arrows). (I-L) Detection of cranial motoneurons in control Tg(isl1:GFP) embryos and Tg(isl1:GFP) embryos that received cdx1aMO and cdx4MO at 48 hpf. Bright field images (J,L). The position of trigeminal (V), facial (VII) and vagal (X) motor nuclei was indicated. A cluster of the GFP+ neurons with their axons were detected in the posteriormost region of cdx1a/4 morphant embryos (arrowhead, K). Scale bars: 500 μm in A,B,E,F; 100 μm in C,D,G,H,I,K.

Expression Data

Gene:	alcama
Antibody:	zn-5
Fish:	WT WT + MO1-cdx1a + MO1-cdx4
Knockdown Reagents:	MO1-cdx1a MO1-cdx4
Anatomical Terms:	hindbrain spinal cord
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-cdx1a + MO1-cdx4
Knockdown Reagents:	MO1-cdx1a MO1-cdx4
Observed In:	hindbrain neuron post-vent region whole organism
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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