FIGURE
            Fig. S1
                
                    
                        Fig. S1
                    
                    
                
                
            
        
        
    
        
            
            
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 Injection of a splice-blocking morpholino results in the loss of lef1 mRNA transcripts. (A) Diagram of lef1 gene structure indicating the location of the lef1 MO target at the exon 7 splice donor site. Introns are not to scale. (B) RT-PCR analysis demonstrates that injection of the lef1 MO into zebrafish embryos at the one-cell stage results in the production of aberrantly spliced forms at 12 hpf and the loss of lef1 mRNA after 18 hpf.  | 
    
                
                    
                        Expression Data
                    
                    
                
                
            
        
        
    
        
            
            
    | Gene: | |
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| Fish: | |
| Knockdown Reagent: | |
| Anatomical Term: | |
| Stage Range: | 1k-cell to Prim-5 | 
                
                    
                        Expression Detail
                    
                    
                
                
            
        
        
    
        
            
                
            
        
    
    
    
                
                    
                        Antibody Labeling
                    
                    
                
                
            
        
        
    
        
            
                
            
        
    
    
    
                
                    
                        Phenotype Data
                    
                    
                
                
            
        
        
    
        
            
            
            
            
    
    
                
                    
                        Phenotype Detail
                    
                    
                
                
            
        
        
    
        
            
                
            
        
    
    
    
                
                    
                        Acknowledgments
                    
                    
                
                
            
        
        
    
        
            
            
                
                    
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