Fig. 6
- ID
- ZDB-FIG-061121-21
- Publication
- Eroglu et al., 2006 - Critical role of Brg1 member of the SWI/SNF chromatin remodeling complex during neurogenesis and neural crest induction in zebrafish
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Brg1-MO-injected embryos exhibit reduced expression of genes critical for neural crest induction. In situ hybridization analyses showing the expression of indicated genes in control-MO-injected (10 ng/embryo) or Brg1-MO-injected (5 or 10 ng/embryo), or yng mutant embryos at 12 hpf or the 15-somite (15s) stages. The expression of foxd3, tfap2a, and snail2 were analyzed. Morphant phenotypes were more than 90%, n = 40. Dorsal view of embryos showing expression of foxd3 (A-D,M,N), tfap2a (E-H), and snail2 (I-L). For the in situ hybridization analyses, a total of 2,000 embryos was injected and 100 embryos were used per probe. No nonspecific hybridization was observed with sense probes (data not shown). Original magnification 40×. |
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Stage Range: | 5-9 somites to 14-19 somites |