ZFIN ID: ZDB-IMAGE-060919-3
Figures for Goishi et al., 2006

Genes:

Figure Caption/Comments:

Fig. S2 crystallin insolubility and rescue in cloches5. (A) Western blot: total clochem39 and cloches5 embryo extracts were analyzed by western blotting with anti-αA-crystallin antibody. (B) Zebrafish embryo (2-4 dpf) mRNA levels were analyzed by semi-quantitative RT-PCR. Wild-type versus cloches5 embryo gene expression is shown for αA-crystallin and γ-crystallin. ef1a was used as a loading control. (C) αA-crystallin overexpression prevents γ-crystallin insolubility. Zebrafish embryos (2.5 dpf) were lysed detergent-free in 20 mM Tris-HCl, pH 7.5, and centrifuged. Soluble (top) and insoluble (bottom) fractions were separated, and were each analyzed by western blot with anti-γ-crystallin antibody. Two independent experiments using cloches5 were performed.

Figure Data:
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.