ZFIN ID: ZDB-FIG-060501-5
Miller-Bertoglio et al., 1997 - Differential regulation of chordin expression domains in mutant zebrafish. Developmental Biology   192:537-550 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Shield to 90%-epiboly

Fig. 4 Altered dorsolateral chordin expression domains in dorsalized and ventralized zebrafish mutants. (A – F) Expression of chordin in dorsalized swr mutants (B, D, F) was expanded ventrally (arrowheads) compared to WT sibs (A, C, E). A and B are animal pole views and C and D are dorsal views at 75% epiboly. E and F are vegetal pole views at 90% epiboly. (G) At shield stage, dorsal organizer expression of chordin was indistinguishable in sbn mutants and WT siblings (dorsal view) and in embryos from intercrosses of all other dorsalizing mutations examined (not shown). (H – L) Animal pole views. Expression was ventrally expanded (arrowheads) compared to WT (H, K) in both sbn (I and J) and snh (L) mutants at 75% epiboly. As described in the text, all embryos from sbn intercrosses had altered expression patterns which fell into two classes (I was the pattern of two-thirds and J was the pattern of one-third of embryos). (M – Q) Dorsal views. (M) chordin expression was indistinguishable in weakly ventralized mes mutants and WT siblings at 75% epiboly. (N, O) However, expression was decreased overall in ventralized ogo mutant embryos (O) compared to WT sibs (N) at 90% epiboly. (P, Q) Dorsolateral domains of chordin expression (arrowheads) were also more dorsally restricted in stl mutants (Q) compared to WT siblings (P) at 80% epiboly. Asterisks indicate the dorsal midline. Scale bar = 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
chrd Df(Chr25:ogo_b180)b180/b180(AB) standard conditions 75%-epiboly axial chorda mesoderm ISH
75%-epiboly axis ISH
75%-epiboly ectoderm ISH
75%-epiboly margin ISH
75%-epiboly prechordal plate ISH
75%-epiboly presumptive mesoderm ISH
Df(Chr25:szl)m60/m60(HK) standard conditions 90%-epiboly axis ISH
90%-epiboly margin ISH
WT standard conditions Shield shield ISH
75%-epiboly axial chorda mesoderm ISH
75%-epiboly axis ISH
75%-epiboly margin ISH
75%-epiboly prechordal plate ISH
75%-epiboly presumptive mesoderm ISH
90%-epiboly axial chorda mesoderm ISH
90%-epiboly axis ISH
90%-epiboly ectoderm ISH
90%-epiboly margin ISH
90%-epiboly prechordal plate ISH
90%-epiboly presumptive mesoderm ISH
bmp2bta72a/ta72a(TU) standard conditions 75%-epiboly axial chorda mesoderm ISH
75%-epiboly margin ISH
75%-epiboly prechordal plate ISH
75%-epiboly presumptive mesoderm ISH
90%-epiboly axial chorda mesoderm ISH
90%-epiboly ectoderm ISH
90%-epiboly margin ISH
90%-epiboly prechordal plate ISH
90%-epiboly presumptive mesoderm ISH
bmp7aty68a/ty68a standard conditions 75%-epiboly axial chorda mesoderm ISH
75%-epiboly axis ISH
75%-epiboly margin ISH
75%-epiboly prechordal plate ISH
75%-epiboly presumptive mesoderm ISH
smad5dtc24/dtc24(TU) standard conditions Shield shield ISH
75%-epiboly axial chorda mesoderm ISH
75%-epiboly axis ISH
75%-epiboly margin ISH
75%-epiboly prechordal plate ISH
75%-epiboly presumptive mesoderm ISH
szltm305/tm305 standard conditions 75%-epiboly axial chorda mesoderm ISH
75%-epiboly axis ISH
75%-epiboly margin ISH
75%-epiboly prechordal plate ISH
75%-epiboly presumptive mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 192, Miller-Bertoglio, V.E., Fisher, S., Sanchez, A., Mullins, M.C., and Halpern, M., Differential regulation of chordin expression domains in mutant zebrafish, 537-550, Copyright (1997) with permission from Elsevier. Full text @ Dev. Biol.