PUBLICATION

Differential regulation of chordin expression domains in mutant zebrafish

Authors
Miller-Bertoglio, V.E., Fisher, S., Sanchez, A., Mullins, M.C., and Halpern, M.
ID
ZDB-PUB-980205-19
Date
1997
Source
Developmental Biology   192: 537-550 (Journal)
Registered Authors
Fisher, Shannon, Halpern, Marnie E., Mullins, Mary C.
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Cell Differentiation
  • Cloning, Molecular
  • Drosophila Proteins*
  • Drosophila melanogaster/genetics
  • Embryo, Nonmammalian/metabolism
  • Embryonic Induction
  • Gastrula/metabolism
  • Gene Expression Regulation, Developmental*
  • Genes*
  • Glycoproteins/biosynthesis*
  • Glycoproteins/genetics
  • Glycoproteins/physiology
  • Insect Proteins/physiology
  • Intercellular Signaling Peptides and Proteins*
  • Mesoderm/physiology
  • Molecular Sequence Data
  • Morphogenesis/genetics
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Xenopus laevis/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed
9441687 Full text @ Dev. Biol.
Abstract
Patterning along the dorsal-ventral (D-V) axis of Xenopus and Drosophila embryos is believed to occur through a conserved molecular mechanism, with homologous proteins Chordin and Short gastrulation (Sog) antagonizing signaling by bone morphogenetic protein 4 (BMP-4) and Decapentaplegic (Dpp), respectively. We have isolated a zebrafish gene that is highly homologous to chordin and sog within cysteine-rich domains and exhibits conserved aspects of expression and function. As in Xenopus embryos, zebrafish chordin is expressed in the organizer region and transiently in axial mesoderm. Injection of zebrafish chordin mRNA to the ventral side of Xenopus embryos induced secondary axes. Ectopic overexpression in zebrafish resulted in an expansion of paraxial mesoderm and neurectoderm at the expense of more lateral and ventral derivatives, producing a range of defects similar to those of dorsalized zebrafish mutants (Mullins et al., 1996). In accordance with the proposed function of chordin in D-V patterning, dorsalized zebrafish mutants showed expanded domains of chordin expression by midgastrulation, while some ventralized mutants had reduced expression; however, in all mutants examined, early organizer expression was unaltered. In contrast to Xenopus, zebrafish chordin is also expressed in paraxial mesoderm and ectoderm and in localized regions of the developing brain, suggesting that there are additional roles for chordin in zebrafish embryonic development. Surprisingly, paraxial mesodermal expression of chordin appeared unaltered in spadetail mutants that later lack trunk muscle (Kimmel et al., 1989), while axial mesodermal expression was affected. This finding reveals an unexpected function for spadetail in midline mesoderm and in differential regulation of chordin expression during gastrulation.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes