FIGURE

Fig. 5

ID

ZDB-FIG-060322-5

Publication

Seiliez et al., 2006 - FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation

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Fig. 5

Inhibition of gsc and foxA3 results in an upregulation of Wnt signaling at the dorsal margin. Compared to wild-type (A), embryos injected with wnt8a DNA (B) or inactivation of dkk1 by morpholino knock-down (C) results in deletion of anterior head. (D–F) Expression of wnt8a at shield stage in WT (D), gsc (E) and gsc/foxa3 (F) knock-down embryos. Arrowheads indicate the dorsal borders of wnt8a expression territory. (G–I) Expression of frzb at shield stage in WT (G), gsc (H) and gsc/foxa3 (I) knock-down embryos. (J, K) Expression of dkk1 at shield stage in WT (J) and gsc/foxa3 knock-down (K) embryos. Arrows indicate the dorsal most extent domain of dkk1 expression at the margin. (L) Expression of wnt8a at shield stage in an embryo injected with gsc RNA at the 32 cell stage in one ventro-lateral blastomere. Arrowheads indicate the limit of wnt8a expression at the gastrula margin. (A, B) 96 hpf, (C) 48 hpf, (D–L) shield stage. (A–C, G–I) Lateral view, (D–F) dorsal view, (J–L) animal pole view dorsal to the right.

Expression Data

Genes:	dkk1b frzb wnt8a
Fish:	WT WT + MO1-gsc WT + MO1-foxa3 + MO1-gsc
Knockdown Reagents:	MO1-foxa3 MO1-gsc
Anatomical Term:	margin
Stage:	Shield

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dkk1b
Knockdown Reagent:	MO1-dkk1b
Observed In:	head head morphogenesis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 290(1), Seiliez, I., Thisse, B., and Thisse, C., FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation, 152-163, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.