FIGURE

Fig. 4

ID

ZDB-FIG-060322-4

Publication

Seiliez et al., 2006 - FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation

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Fig. 4

Inhibition of gsc plus foxA3 posteriorizes the ectoderm. (A–F) Expression of the presumptive epidermal marker foxi1 and the presumptive MHB marker pax2.1 in WT (A, D), gsc (B, E) and gsc/foxA3 (C, F) knock-down embryos. The region between the animal pole (red star) and the presumptive MHB (arrowhead) corresponding to the presumptive forebrain and midbrain is reduced in gsc and almost absent in gsc/foxA3 knock-down embryos. (G, H) Expression of hoxa1 in WT (G) is expanded anteriorly (red arrow) in gsc/foxA3 knock-down embryos (H). The embryos are at 80% epiboly. (A–C, G and H left) Lateral views and (D–F) animal pole view, dorsal to the right. (G and H right) Dorsal views anterior to the top.

Expression Data

Genes:	foxi1 hoxb1b pax2a
Fish:	WT WT + MO1-gsc WT + MO1-foxa3 + MO1-gsc
Knockdown Reagents:	MO1-foxa3 MO1-gsc
Anatomical Terms:	ectoderm presumptive midbrain hindbrain boundary presumptive neural plate
Stage:	75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 290(1), Seiliez, I., Thisse, B., and Thisse, C., FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation, 152-163, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.