ZFIN ID: ZDB-FIG-060303-7
Milan et al., 2006 - Notch1b and neuregulin are required for specification of central cardiac conduction tissue. Development (Cambridge, England)   133(6):1125-1132 Full text @ Development
Anatomical Terms:
Stage: Long-pec
Observed In:
Stage: Long-pec

Fig. 3 Endocardial signaling is required for the development of AV conduction tissue. (A) Calcium activation map of a single representative cardiac cycle in cloche mutant embryos at 48 hpf. Isochronal lines (black) obtained by fluorescence microscopy are superimposed on a maximum intensity projection of the same heart and demonstrate the failure of AV conduction tissue development in the absence of endocardium. (B) Lack of AV conduction block in cloche embryos compared with wild-type siblings is demonstrated by atrial pacing (white bars) and terfenadine exposure (gray bars). Inset of contemporaneous recordings of atrial and ventricular contraction using intensity-time plots from regions over the respective chambers in a terfenadine treated cloche embryo demonstrating 1:1 AV conduction. (C,D). In situ expression patterns of notch1b (C) and bmp4 (D) in cloche embryos at 48 hpf. Despite overstaining in the brain there is no evidence of notch1b signal in the heart (C). In D, the black arrowhead indicates the location of the AV ring; the white arrowhead denotes intense expression of bmp4 at the sinus venosus.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmp4 npas4lDf(Chr13:lycat)m39 standard conditions Long-pec Not Detected atrioventricular ring ISH
Long-pec Not Detected heart ISH
Long-pec sinus venosus cardiac muscle cell ISH
notch1b npas4lDf(Chr13:lycat)m39 standard conditions Long-pec Not Detected atrioventricular ring ISH
Long-pec brain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
npas4lDf(Chr13:lycat)m39 standard conditions Long-pec atrioventricular ring bmp4 expression absent, abnormal
Long-pec atrioventricular ring notch1b expression absent, abnormal
Long-pec cardiac conduction rate, abnormal
Long-pec heart bmp4 expression absent, abnormal
Long-pec sinus venosus cardiac muscle cell bmp4 expression increased amount, abnormal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development