ZFIN ID: ZDB-FIG-060303-11
Milan et al., 2006 - Notch1b and neuregulin are required for specification of central cardiac conduction tissue. Development (Cambridge, England)   133(6):1125-1132 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 7 Notch is required for the induction of AV conduction tissue. (A) notch1b knockdown embryos fail to develop an AV conduction delay, as shown in this calcium activation map of a single cardiac cycle in a notch1b morphant at 48 hpf. Isochronal lines (50 mseconds) obtained by fluorescence microscopy are superimposed on a maximum intensity projection image. (B) notch1b morphants fail to develop an AV conduction block on treatment with terfenadine, as seen in this histogram of the proportion of embryos developing 2:1 AV block at 48 hpf. Error bars indicate s.e.m. (C) neuregulin morphants exhibit normal expression of notch1b, as seen in this in situ hybridization. Arrowhead indicates cardiac expression in the AV ring. (D) notch morphants exhibit normal expression of neuregulin, as seen in this in situ hybridization. Arrowhead indicates cardiac expression in the AV ring.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
notch1b WT + MO1-nrg1 standard conditions Long-pec atrioventricular ring ISH
Long-pec brain ISH
nrg1 WT + MO2-notch1b standard conditions Long-pec atrioventricular ring ISH
Long-pec brain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-nrg1 standard conditions Long-pec atrioventricular ring notch1b expression amount, ameliorated
Long-pec atrioventricular ring notch1b expression position, ameliorated
WT + MO2-notch1b standard conditions Long-pec cardiac conduction rate, abnormal
WT + MO2-notch1b standard conditions Long-pec atrioventricular ring nrg1 expression amount, ameliorated
Long-pec atrioventricular ring nrg1 expression position, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development