ZFIN ID: ZDB-FIG-060303-19
Stafford et al., 2006 - Retinoids signal directly to zebrafish endoderm to specify insulin-expressing {beta}-cells. Development (Cambridge, England)   133(5):949-956 Full text @ Development
Knockdown Reagent:
Anatomical Terms:
Stage Range: Bud to Prim-5

Fig. 2 Pancreas development is dependent on RAR function. (A) rara2a expression in dorsal view at 10 hpf. (B) rara2b expression in dorsal view at 10 hpf; arrowhead indicates the approximate AP position where the pancreas will develop. (C) insulin is expressed at 24 hpf following morpholino knock-down of RARα2b (indistinguishable from controls; data not shown). (D-F) rara2b expression in endoderm cells; examples are indicated by asterisks. Confocal images of rara2b expression (red) at 10 hpf, SOX32-positive donor-derived endoderm is labeled with fluorescein (green). Approximate area of image is boxed in B. (G) DN-RARα2a mRNA blocks insulin expression in a dose-dependent fashion (concentrations injected in ng/┬Ál). (H) Histogram showing number of insulin-expressing cells in uninjected control embryos (n=44) compared with embryos injected with RARα2a-MO (n=11), RARα2b-MO (n=12), RARα-MO (n=12), RARα2a-MO plus RARα2b-MO (n=10), RARα2b-MO plus RARα-MO (n=7), DN-RARα2a mRNA (125 ng/ml; n=11) and DN-RARα2a mRNA (125 ng/ml) plus RARα2b-MO (RAR knock-down, RKD; n=85). Error bars indicate standard deviation. (I) Co-injection of DN-RARα2a mRNA and RARα2b-MO blocks insulin expression.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ins WT + MO1-rarab standard conditions Prim-5 pancreas primordium ISH
raraa WT standard conditions Bud unspecified ISH
rarab WT standard conditions Bud endoderm ISH
Bud mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development