FIGURE

Fig. 3

ID

ZDB-FIG-060303-20

Publication

Stafford et al., 2006 - Retinoids signal directly to zebrafish endoderm to specify insulin-expressing {beta}-cells

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Fig. 3

The DN-RARα2a acts in a dominant negative fashion. (A) DN-RARα2a is able to block induction of an RA-responsive luciferase reporter gene (tk-[βRARE]₂-luc). Co-injection of DN-RARα2a RNA completely abolishes the response of the reporter gene to RA. Graph shows fold-induction relative to reporter alone; six embryos were used for each experimental condition and assays were carried out in duplicate and averaged. (B-E) hoxa4a expression, 10 hpf, lateral view. (B) Normal embryo. (C) hoxa4a expression is upregulated and extended towards the anterior after 1 hour treatment with RA. (D) DN-RAR mRNA injection blocks hoxa4a expression. (E) DN-RAR blocks the ability of RA to activate hoxa4a expression.

Expression Data

Gene:	hoxa4a
Fish:	WT
Condition:	chemical treatment: retinoic acid
Anatomical Term:	unspecified
Stage:	Bud

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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