ZFIN ID: ZDB-FIG-050819-8
Elworthy et al., 2005 - Phox2b function in the enteric nervous system is conserved in zebrafish and is sox10-dependent. Mechanisms of Development   122(5):659-669 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-15 to Pec-fin
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-15 to Pec-fin

Fig. 7 sox10 mutants show severe and early defects in enteric nervous system development. Lateral views (A, C, E, G, I, K, M, O, Q, S, U, W, Y, AA, AC, AE, AG, AI) or transverse sections (B, D, F, H, J, L, N, P, R, T, V, X, Z, AB, AD, AF, AH, AJ) of whole-mount in situ hybridizations of anterior trunk at 35 (A–L), 45 (M–X) and 60 hpf (Y–AJ) for enteric nervous system markers sox10 (A, B, G, H, M, N, S, T, Y, Z, AE, AF), phox2b (C, D, I, J, O, P, U, V, AA, AB, AG, AH) or ret (E, F, K, L, Q, R, W, X, AC, AD, AI, AJ) in wild-type (A–F, M–R, Y–AD) and sox10 (cls) mutant (G–L, S–X, AE–AJ) embryos. At all stages, wild-type ENS progenitors express all three genes (arrows), whereas sox10 mutants show occasional sox10-positive cells in this location, but do not express phox2b nor ret. As an internal positive control, note that expression of phox2b in the hindbrain (*, C, D, I, J) and ret in the ventral neural tube (#, E, K) is not affected. Note too that in wild-type embryos ret is expressed in both a chain of ENS progenitor cells (arrow and inset in E, Q and AC) and more medially in the tightly packed cells of the forming pronephros (partially out of focus in this focal plane; arrowhead, E, Q); the former is absent in sox10 mutants, whereas the latter is unaffected (K, W, AI). ENS progenitor marker expression extends more posteriorly along the gut with increasing age (e.g. compare C, O, AA). sox10 expression consistently extends more posteriorly than the other two markers at equivalent stages (e.g. compare A, C, E). Transverse sections reveal changes in progenitor distribution around gut primordium with age. ENS progenitors at 35 hpf initially lie in contact with gut primordium (arrow, B), forming two longitudinal columns migrating along the gut, but have spread around the developing gut by 60 hpf (Z). Note the identical positioning of sox10, phox2b and ret positive progenitors with respect to the developing gut (e.g. N, P, R). ENS progenitors are not usually seen in sections of cls mutant embryos (H, J, L, T, V, X, AF, AH, AJ), although expression in CNS remains. Interestingly, sox10 and phox2b expression is prominent and overlapping in the pectoral fin mesenchyme (p) of wild-types (N, P), whereas phox2b appears to be strongly reduced in cls mutants (T, V). NB All whole-mount embryos treated with PTU to inhibit melanin synthesis. g, gut primordium.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
phox2bb WT chemical treatment: N-phenylthiourea Prim-15 hindbrain ISH
Prim-15 presumptive enteric nervous system ISH
High-pec mesenchyme pectoral fin ISH
High-pec presumptive enteric nervous system ISH
Pec-fin enteric nervous system ISH
sox10m618/m618(AB) chemical treatment: N-phenylthiourea Prim-15 hindbrain ISH
Prim-15 Not Detected presumptive enteric nervous system ISH
High-pec mesenchyme pectoral fin ISH
High-pec Not Detected presumptive enteric nervous system ISH
Pec-fin Not Detected enteric nervous system ISH
ret WT chemical treatment: N-phenylthiourea Prim-15 central nervous system ISH
Prim-15 presumptive enteric nervous system ISH
Prim-15 pronephros ISH
High-pec presumptive enteric nervous system ISH
High-pec pronephros ISH
Pec-fin enteric nervous system ISH
sox10m618/m618(AB) chemical treatment: N-phenylthiourea Prim-15 central nervous system ISH
Prim-15 Not Detected presumptive enteric nervous system ISH
Prim-15 pronephros ISH
High-pec Not Detected presumptive enteric nervous system ISH
High-pec pronephros ISH
Pec-fin Not Detected enteric nervous system ISH
sox10 WT chemical treatment: N-phenylthiourea Prim-15 presumptive enteric nervous system ISH
High-pec mesenchyme pectoral fin ISH
High-pec presumptive enteric nervous system ISH
Pec-fin enteric nervous system ISH
sox10m618/m618(AB) chemical treatment: N-phenylthiourea Prim-15 presumptive enteric nervous system ISH
High-pec mesenchyme pectoral fin ISH
High-pec presumptive enteric nervous system ISH
Pec-fin enteric nervous system ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sox10m618/m618(AB) chemical treatment: N-phenylthiourea Prim-15 presumptive enteric nervous system aplastic, abnormal
High-pec presumptive enteric nervous system aplastic, abnormal
Pec-fin presumptive enteric nervous system aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 122(5), Elworthy, S., Pinto, J.P., Pettifer, A., Cancela, M.L., and Kelsh, R.N., Phox2b function in the enteric nervous system is conserved in zebrafish and is sox10-dependent, 659-669, Copyright (2005) with permission from Elsevier. Full text @ Mech. Dev.