PUBLICATION
Phox2b function in the enteric nervous system is conserved in zebrafish and is sox10-dependent
- Authors
- Elworthy, S., Pinto, J.P., Pettifer, A., Cancela, M.L., and Kelsh, R.N.
- ID
- ZDB-PUB-050413-8
- Date
- 2005
- Source
- Mechanisms of Development 122(5): 659-669 (Journal)
- Registered Authors
- Elworthy, Stone, Kelsh, Robert
- Keywords
- Phox2b; Enteric neuron; Colorless; Sox10; Fate specification; Progenitors; Stem cells; Neural crest; Melanophore
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Carrier Proteins/genetics*
- Carrier Proteins/metabolism
- Carrier Proteins/physiology*
- Central Nervous System/embryology
- Chromosome Mapping
- Enteric Nervous System/embryology*
- Gene Expression Regulation, Developmental*
- High Mobility Group Proteins/genetics*
- High Mobility Group Proteins/metabolism
- High Mobility Group Proteins/physiology*
- Homeodomain Proteins/metabolism
- Homeodomain Proteins/physiology*
- In Situ Hybridization
- Melanophores/metabolism
- Microscopy, Fluorescence
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Neural Crest/metabolism
- Neurons/metabolism
- Phenotype
- Phylogeny
- SOXE Transcription Factors
- Sequence Homology, Amino Acid
- Stem Cells/metabolism
- Time Factors
- Transcription Factors/metabolism
- Transcription Factors/physiology*
- Zebrafish
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- PubMed
- 15817223 Full text @ Mech. Dev.
Citation
Elworthy, S., Pinto, J.P., Pettifer, A., Cancela, M.L., and Kelsh, R.N. (2005) Phox2b function in the enteric nervous system is conserved in zebrafish and is sox10-dependent. Mechanisms of Development. 122(5):659-669.
Abstract
Zebrafish lacking functional sox10 have defects in non-ectomesenchymal neural crest derivatives including the enteric nervous system (ENS) and as such provide an animal model for human Waardenburg Syndrome IV. Here, we characterize zebrafish phox2b as a functionally conserved marker of the developing ENS. We show that morpholino-mediated knockdown of Phox2b generates fish modeling Hirschsprung disease. Using markers, including phox2b, we investigate the ontogeny of the sox10 ENS phenotype. As previously shown for melanophore development, ENS progenitor fate specification fails in these mutant fish. However, in addition, we trace back the sox10 mutant ENS defect to an even earlier time point, finding that most neural crest cells fail to migrate ventrally to the gut primordium.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping