FIGURE

Fig. 3

ID

ZDB-FIG-050819-3

Publication

Nechiporuk et al., 2005 - Endoderm-derived Fgf3 is necessary and sufficient for inducing neurogenesis in the epibranchial placodes in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Fgf3 is necessary for inducing neurogenesis in EB placodes. Embryos injected with morpholino directed against fgf3 (A-C, right) and uninjected controls (A-C, left) were processed for in-situ hybridization with ngn1, phox2a and phox2b at 36 hpf, immunolabeling with Hu antibody at 80 hpf and Zn-5 antibody at 36 hpf, and TUNEL assay at 26 hpf. All whole-mount sections show lateral views. Anterior is left. (A) ngn1, phox2a, phox2b and Hu expression were absent in the glossopharyngeal and small vagal placodes and ganglia and significantly reduced in the facial and large vagal placodes and ganglia (arrows). (B) Zn-5 staining demonstrated that pharyngeal pouches were not affected by fgf3-MO1 injection (arrowheads). (C) TUNEL assay revealed dying cells in the ectoderm of the fgf3-morphants (arrows). Insets show transverse sections through the facial placode. Scale bars: 50 µm. Abbreviations are as in Fig. 1.

Expression Data

Genes:	alcama neurog1 phox2a phox2bb
Fish:	WT WT + MO4-fgf3
Knockdown Reagent:	MO4-fgf3
Anatomical Terms:	facial ganglion glossopharyngeal ganglion pharyngeal pouch vagal ganglion 1 vagal ganglion 2 vagal ganglion 3 vagal ganglion 4
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development