ZFIN Figure: Sumanas et al., 2005, Fig. 7

ZFIN ID: ZDB-FIG-050723-4

ZFIN ID: ZDB-FIG-050723-4

Sumanas et al., 2005 - 15-Zinc finger protein Bloody Fingers is required for zebrafish morphogenetic movements during neurulation. Developmental Biology 283(1):85-96 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	crestin gata3 her4.2 krt97
Fish:	WT WT + MO2-blf
Knockdown Reagent:	MO2-blf
Anatomical Terms:	neural crest neural plate neural rod neural tube non neural ectoderm periderm
Stage Range:	1-4 somites to 14-19 somites

Fig. 7 In situ hybridization analysis of neural and ectodermal markers in blf morphants, injected with 2 ng of MO2. Dorsal view, anterior is to the left, except where noted. (A, B, E, F) Hairy-related transcription factor her4 labels a subset of neural cells at the 3-somite (A, B) and 6-somite (E, F) stages. Note the expanded neural plate (arrowheads) in blf morphants (B, F), compared with uninjected control embryos (A, E). (C, D, G, H) GATA-family transcription factor gata3 labels non-neural ectoderm at the 3-somite (C, D) and 6-somite (G, H) stages. Note that the gap in gata3 expression which corresponds to the neural plate (arrowheads), is wider in blf morphants (D, H), compared with uninjected control embryos (C, G). Additional gata3-expressing cells are observed at the midline of blf morphants (arrow, H). (I, J, M, N) Her4 expression labels neural tube at the 10-somite (I, J) and 15-somite (M, N) stages. Note the bilaterally split neural tube in blf morphants (J, N), compared with uninjected control embryos (I, M). (K, L, O, P) Cytokeratin E7 expression labels epidermal cells at the 10-somite (K, L) and 15-somite (O, P) stages. Note the strong enrichment in cytokeratin expression in the delaminating cells of dorsal posterior ectoderm in blf morphants (arrows in L, P), compared with uninjected control embryos (K, O). (Q, R) Crestin expression labels neural crest cells at the 16-somite stage. Note two bilateral stripes of neural crest cells in blf morphants (R) compared with the single stripe in control uninjected embryos (Q). (S, T) Crestin expression, 16-somite stage. Note the absence of the intersomitic bands of migrating neural crest cells (arrow) in blf morphants (T), compared with uninjected control embryos (S).

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
crestin	WT	standard conditions	14-19 somites	neural crest	ISH
	WT + MO2-blf	standard conditions	14-19 somites	neural crest	ISH
gata3	WT	standard conditions	1-4 somites	non neural ectoderm	ISH
			5-9 somites	non neural ectoderm	ISH
	WT + MO2-blf	standard conditions	1-4 somites	non neural ectoderm	ISH
			5-9 somites	non neural ectoderm	ISH
her4.2	WT	standard conditions	1-4 somites	neural plate	ISH
			5-9 somites	neural plate	ISH
			10-13 somites	neural rod	ISH
			14-19 somites	neural tube	ISH
	WT + MO2-blf	standard conditions	1-4 somites	neural plate	ISH
			5-9 somites	neural plate	ISH
			10-13 somites	neural rod	ISH
			14-19 somites	neural tube	ISH
krt97	WT	standard conditions	10-13 somites	periderm	ISH
			14-19 somites	periderm	ISH
	WT + MO2-blf	standard conditions	10-13 somites	periderm	ISH
			14-19 somites	periderm	ISH

Antibody Labeling Details No data available

Phenotype Details No data available

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Developmental Biology, 283(1), Sumanas, S., Zhang, B., Dai, R., and Lin, S., 15-Zinc finger protein Bloody Fingers is required for zebrafish morphogenetic movements during neurulation, 85-96, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 283(1), Sumanas, S., Zhang, B., Dai, R., and Lin, S., 15-Zinc finger protein Bloody Fingers is required for zebrafish morphogenetic movements during neurulation, 85-96, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.