ZFIN ID: ZDB-FIG-050723-3
Sumanas et al., 2005 - 15-Zinc finger protein Bloody Fingers is required for zebrafish morphogenetic movements during neurulation. Developmental Biology   283(1):85-96 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5

Fig. 5 In situ hybridization analysis of axial markers in blf morphants, injected with 2 ng of MO2. Anterior is to the left in all panels. (A, B) Notochord marked by no tail (ntl) expression at the 10-somite stage. Note the shortened, thickened, and undulating notochord in blf morphants (B), compared with control uninjected embryos (A). (C, D) Sonic hedgehog (shh) marks the notochord (n) and the floorplate (fp) at the 22-somite stage. Note the undulating notochord and floorplate in blf morphants (D), compared with control uninjected embryos (C). (E, F) myod expression marks paraxial somitic mesoderm at the 10-somite stage. Note wider and compressed somites as well as undulations in adaxial stripes of myod-expressing cells in blf morphants (F), compared with control uninjected embryos (E). (G, H) Expression of spondin1b (spon1b) marks the floorplate at the 22-somite stage. Note severe undulations (arrows) and delaminating cells (arrowhead) in blf morphants (H), compared to uninjected control embryos (G). Inset, high-magnification dorsal view of the floorplate. Note the wider and disorganized floorplate in the inset (H). A cluster of spon1b-expressing cells failed to converge to the midline (arrowhead, inset in H). (I, J) Expression of the forkhead family transcription factor foxA1 (fkd7) marks the floorplate (fp), the hypochord (hc), and endoderm at 24 hpf. Note undulations of the floorplate and the hypochord in blf morphants (J), compared with uninjected controls (I).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxa1 WT standard conditions Prim-5 endoderm ISH
Prim-5 floor plate ISH
Prim-5 hypochord ISH
WT + MO2-blf standard conditions Prim-5 endoderm ISH
Prim-5 floor plate ISH
Prim-5 hypochord ISH
myod1 WT standard conditions 10-13 somites segmental plate ISH
WT + MO2-blf standard conditions 10-13 somites segmental plate ISH
shha WT standard conditions 20-25 somites floor plate ISH
20-25 somites notochord ISH
WT + MO2-blf standard conditions 20-25 somites floor plate ISH
20-25 somites notochord ISH
spon1b WT standard conditions 20-25 somites floor plate ISH
WT + MO2-blf standard conditions 20-25 somites floor plate ISH
tbxta WT standard conditions 10-13 somites notochord ISH
WT + MO2-blf standard conditions 10-13 somites notochord ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 283(1), Sumanas, S., Zhang, B., Dai, R., and Lin, S., 15-Zinc finger protein Bloody Fingers is required for zebrafish morphogenetic movements during neurulation, 85-96, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.