ZFIN Lab: University of Antwerp zebrafishlab - Dries Knapen

ZFIN ID: ZDB-LAB-121116-1

University of Antwerp zebrafishlab - Dries Knapen

PI/Director:	Knapen, Dries
Co-PI / Senior Researcher:	Blust, Ronny Van Cruchten, Steven
Contact Person:	Knapen, Dries
Email:	dries.knapen@ua.ac.be
URL:	http://www.zebrafishlab.be
Address:	University of Antwerp Zebrafishlab University of Antwerp Department of Veterinary Sciences Campus Drie Eiken Universiteitsplein 1, UC.173 2610 Wilrijk Belgium
Country:	Belgium
Phone:	+3232652724
Fax:	+3232652729
Line Designation:	None assigned

GENOMIC FEATURES ORIGINATING FROM THIS LAB No data available

STATEMENT OF RESEARCH INTERESTS

LAB MEMBERS

Hagenaars, An Post-Doc	Vergauwen, Lucia Post-Doc	Bagci, Enise Graduate Student
Van Den Plas, Pieter Graduate Student	Verbueken, Evy Graduate Student	Dardenne, Freddy Research Staff
Joosen, Steven Fish Facility Staff

ZEBRAFISH PUBLICATIONS OF LAB MEMBERS

van den Boom, R., Vergauwen, L., Koedijk, N., da Silva, K.M., Covaci, A., Knapen, D. (2023) Combined western diet and bisphenol A exposure induces an oxidative stress-based paraoxonase 1 response in larval zebrafish. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 274:109758109758

Van Dingenen, I., Vergauwen, L., Haigis, A.C., Blackwell, B.R., Stacy, E., Villeneuve, D.L., Knapen, D. (2023) Deiodinase inhibition impairs the formation of the three posterior swim bladder tissue layers during early embryonic development in zebrafish. Aquatic toxicology (Amsterdam, Netherlands). 261:106632106632

Braeuning, A., Balaguer, P., Bourguet, W., Carreras-Puigvert, J., Feiertag, K., Kamstra, J.H., Knapen, D., Lichtenstein, D., Marx-Stoelting, P., Rietdijk, J., Schubert, K., Spjuth, O., Stinckens, E., Thedieck, K., van den Boom, R., Vergauwen, L., von Bergen, M., Wewer, N., Zalko, D. (2023) Development of new approach methods for the identification and characterization of endocrine metabolic disruptors-a PARC project. Frontiers in toxicology. 5:12125091212509

Philippe, C., Vergauwen, L., Huyghe, K., De Boeck, G., Knapen, D. (2023) Chronic handling stress in zebrafish Danio rerio husbandry. Journal of Fish Biology. 103(2):367-377

Gölz, L., Baumann, L., Pannetier, P., Braunbeck, T., Knapen, D., Vergauwen, L. (2022) AOP Report: Thyroperoxidase Inhibition Leading to Altered Visual Function in Fish via Altered Retinal Layer Structure. Environmental toxicology and chemistry. 41(11):2632-2648

da Silva, K.M., Iturrospe, E., van den Boom, R., van de Lavoir, M., Robeyns, R., Vergauwen, L., Knapen, D., Cuykx, M., Covaci, A., van Nuijs, A.L.N. (2022) Lipidomics profiling of zebrafish liver through untargeted liquid chromatography-high resolution mass spectrometry. Journal of separation science. 45(15):2935-2945

Thessen, A.E., Marvel, S., Achenbach, J.C., Fischer, S., Haendel, M.A., Hayward, K., Klüver, N., Könemann, S., Legradi, J., Lein, P., Leong, C., Mylroie, J.E., Padilla, S., Perone, D., Planchart, A., Prieto, R.M., Muriana, A., Quevedo, C., Reif, D., Ryan, K., Stinckens, E., Truong, L., Vergauwen, L., Vom Berg, C., Wilbanks, M., Yaghoobi, B., Hamm, J. (2022) Implementation of Zebrafish Ontologies for Toxicology Screening. Frontiers in toxicology. 4:817999

Hoyberghs, J., Bars, C., Ayuso, M., Van Ginneken, C., Foubert, K., Van Cruchten, S. (2021) DMSO Concentrations up to 1% are Safe to be Used in the Zebrafish Embryo Developmental Toxicity Assay. Frontiers in toxicology. 3:804033

Bars, C., Hoyberghs, J., Valenzuela, A., Buyssens, L., Ayuso, M., Van Ginneken, C., Labro, A.J., Foubert, K., Van Cruchten, S.J. (2021) Developmental Toxicity and Biotransformation of Two Anti-Epileptics in Zebrafish Embryos and Early Larvae. International Journal of Molecular Sciences. 22(23):

Massei, R., Knapen, D., Covaci, A., Blust, R., Mayer, P., Vergauwen, L. (2021) Sublethal Effect Concentrations for Non-Polar Narcosis in the Zebrafish Embryo. Environmental toxicology and chemistry. 40(10):2802-2812

Hoyberghs, J., Bars, C., Pype, C., Foubert, K., Ayuso Hernando, M., Van Ginneken, C., Ball, J., Van Cruchten, S. (2020) Refinement of the zebrafish embryo developmental toxicity assay. MethodsX. 7:101087

Stinckens, E., Vergauwen, L., Blackwell, B.R., Ankley, G.T., Villeneuve, D.L., Knapen, D. (2020) The effect of thyroperoxidase and deiodinase inhibition on anterior swim bladder inflation in the zebrafish. Environmental science & technology. 54(10):6213-6223

Pilehvar, A., Cordery, K.I., Town, R.M., Blust, R. (2020) The synergistic toxicity of Cd(II) and Cu(II) to zebrafish (Danio rerio): Effect of water hardness. Chemosphere. 247:125942

Pilehvar, A., Town, R.M., Blust, R. (2019) The interactive effect of copper and conspecific alarm substances on behavioural responses of zebrafish (Danio rerio). Behavioural brain research. 381:112452

Pilehvar, A., Town, R.M., Blust, R. (2019) The effect of copper on behaviour, memory, and associative learning ability of zebrafish (Danio rerio). Ecotoxicology and environmental safety. 188:109900

Cassar, S., Adatto, I., Freeman, J.L., Gamse, J.T., Iturria, I., Lawrence, C., Muriana, A., Peterson, R., Van Cruchten, S., Zon, L. (2019) Use of Zebrafish in Drug Discovery Toxicology. Chemical Research in Toxicology. 33(1):95-118

Pilehvar, A., Town, R.M., Blust, R. (2019) The effect of thermal pre-incubation and exposure on sensitivity of zebrafish (Danio rerio) to copper and cadmium single and binary exposures. Aquatic toxicology (Amsterdam, Netherlands). 213:105226

Baumann, L., Segner, H., Ros, A., Knapen, D., Vergauwen, L. (2019) Thyroid Hormone Disruptors Interfere with Molecular Pathways of Eye Development and Function in Zebrafish. International Journal of Molecular Sciences. 20(7):

Gabriëls, I.J., Vergauwen, L., De Boevre, M., Van Dongen, S., Blust, R., De Saeger, S., Eeckhout, M., De Loose, M., Knapen, D. (2019) Optimizing the Use of Zebrafish Feeding Trials for the Safety Evaluation of Genetically Modified Crops. International Journal of Molecular Sciences. 20(6)

Michiels, E.D.G., Vergauwen, L., Lai, F.Y., Town, R.M., Covaci, A., van Nuijs, A.L.N., Van Cruchten, S.J., Knapen, D. (2018) Advancing the zebrafish embryo test for endocrine disruptor screening using micro-injection: ethinyl estradiol as a case study. Environmental toxicology and chemistry. 38(3):533-547

Verbueken, E., Bars, C., Ball, J.S., Periz-Stanacev, J., Marei, W.F.A., Tochwin, A., Gabriëls, I.J., Michiels, E.D.G., Stinckens, E., Vergauwen, L., Knapen, D., Van Ginneken, C.J., Van Cruchten, S.J. (2018) From mRNA Expression of Drug Disposition Genes to In Vivo Assessment of CYP-Mediated Biotransformation during Zebrafish Embryonic and Larval Development. International Journal of Molecular Sciences. 19(12)

Vergauwen, L., Cavallin, J.E., Ankley, G.T., Bars, C., Gabriëls, I.J., Michiels, E.D.G., Fitzpatrick, K.R., Periz-Stanacev, J., Randolph, E.C., Robinson, S.L., Saari, T.W., Schroeder, A.L., Stinckens, E., Swintek, J., Van Cruchten, S.J., Verbueken, E., Villeneuve, D.L., Knapen, D. (2018) Gene transcription ontogeny of hypothalamic-pituitary-thyroid axis development in early-life stage fathead minnow and zebrafish. General and comparative endocrinology. 266:87-100

Stinckens, E., Vergauwen, L., Ankley, G.T., Blust, R., Darras, V.M., Villeneuve, D.L., Witters, H., Volz, D.C., Knapen, D. (2018) An AOP-based alternative testing strategy to predict the impact of thyroid hormone disruption on swim bladder inflation in zebrafish. Aquatic toxicology (Amsterdam, Netherlands). 200:1-12

Van Camp, K.A., Baggerman, G., Blust, R., Husson, S.J. (2018) Neuropeptidomic Analysis of Zebrafish Brain. Methods in molecular biology (Clifton, N.J.). 1719:241-246

Saad, M., Bijttebier, S., Matheeussen, A., Verbueken, E., Pype, C., Casteleyn, C., Van Ginneken, C., Maes, L., Cos, P., Van Cruchten, S. (2017) UPLC/MS MS data of testosterone metabolites in human and zebrafish liver microsomes and whole zebrafish larval microsomes. Data in brief. 16:644-648

Pype, C., Verbueken, E., Saad, M.A., Bars, C., Van Ginneken, C.J., Knapen, D., Van Cruchten, S.J. (2017) Antioxidants reduce reactive oxygen species but not embryotoxicity in the metabolic Danio rerio test (mDarT). Reproductive toxicology (Elmsford, N.Y.). 72:62-73

Saad, M., Matheeussen, A., Bijttebier, S., Verbueken, E., Pype, C., Casteleyn, C., Van Ginneken, C., Apers, S., Maes, L., Cos, P., Van Cruchten, S. (2017) In vitro CYP-mediated drug metabolism in the zebrafish (embryo) using human reference compounds. Toxicology in vitro : an international journal published in association with BIBRA. 42:329-336

Michiels, E.D., Vergauwen, L., Hagenaars, A., Fransen, E., Dongen, S.V., Van Cruchten, S.J., Bervoets, L., Knapen, D. (2017) Evaluating Complex Mixtures in the Zebrafish Embryo by Reconstituting Field Water Samples: A Metal Pollution Case Study. International Journal of Molecular Sciences. 18(3)

Verbueken, E., Alsop, D., Saad, M.A., Pype, C., Van Peer, E.M., Casteleyn, C.R., Van Ginneken, C.J., Wilson, J., Van Cruchten, S.J. (2017) In Vitro Biotransformation of Two Human CYP3A Probe Substrates and Their Inhibition during Early Zebrafish Development. International Journal of Molecular Sciences. 18(1)

Van Camp, K.A., Baggerman, G., Blust, R., Husson, S.J. (2017) Peptidomics of the zebrafish Danio rerio: In search for neuropeptides. Journal of proteomics. 150:290-296

Saad, M., Verbueken, E., Pype, C., Casteleyn, C., Ginneken, C.V., Maes, L., Cos, P., Cruchten, S.V. (2016) In vitro CYP1A activity in the zebrafish: temporal but low metabolite levels during organogenesis and lack of gender differences in the adult stage. Reproductive toxicology (Elmsford, N.Y.). 64:50-6

Nelson, K.R., Schroeder, A.L., Ankley, G.T., Blackwell, B.R., Blanksma, C., Degitz, S.J., Flynn, K.M., Jensen, K.M., Johnson, R.D., Kahl, M.D., Knapen, D., Kosian, P.A., Milsk, R.Y., Randolph, E.C., Saari, T., Stinckens, E., Vergauwen, L., Villeneuve, D.L. (2016) Impaired anterior swim bladder inflation following exposure to the thyroid peroxidase inhibitor 2-mercaptobenzothiazole part I: Fathead minnow. Aquatic toxicology (Amsterdam, Netherlands). 173:192-203

Stinckens, E., Vergauwen, L., Schroeder, A.L., Maho, W., Blackwell, B.R., Witters, H., Blust, R., Ankley, G.T., Covaci, A., Villeneuve, D.L., Knapen, D. (2016) Impaired anterior swim bladder inflation following exposure to the thyroid peroxidase inhibitor 2-mercaptobenzothiazole part II: Zebrafish. Aquatic toxicology (Amsterdam, Netherlands). 173:204-17

Houbrechts, A.M., Vergauwen, L., Bagci, E., Van Houcke, J., Heijlen, M., Kulemeka, B., Hyde, D.R., Knapen, D., Darras, V.M. (2016) Deiodinase knockdown affects zebrafish eye development at the level of gene expression, morphology and function. Molecular and Cellular Endocrinology. 424:81-93

Saad, M., Cavanaugh, K., Verbueken, E., Pype, C., Casteleyn, C., Ginneken, C.V., Cruchten, S.V. (2016) Xenobiotic metabolism in the zebrafish: a review of the spatiotemporal distribution, modulation and activity of Cytochrome P450 families 1 to 3. The Journal of Toxicological Sciences. 41:1-11

Vergauwen, L., Schmidt, S.N., Stinckens, E., Maho, W., Blust, R., Mayer, P., Covaci, A., Knapen, D. (2015) A high throughput passive dosing format for the Fish Embryo Acute Toxicity test. Chemosphere. 139:9-17

Pype, C., Verbueken, E., Saad, M.A., Casteleyn, C.R., Van Ginneken, C.J., Knapen, D., Van Cruchten, S.J. (2015) Incubation at 32.5°C and above causes malformations in the zebrafish embryo. Reproductive toxicology (Elmsford, N.Y.). 56:56-63

Bagci, E., Heijlen, M., Vergauwen, L., Hagenaars, A., Houbrechts, A.M., Esguerra, C.V., Blust, R., Darras, V.M., Knapen, D. (2015) Deiodinase Knockdown during Early Zebrafish Development Affects Growth, Development, Energy Metabolism, Motility and Phototransduction. PLoS One. 10:e0123285

Hagenaars, A., Stinckens, E., Vergauwen, L., Bervoets, L., Knapen, D. (2014) PFOS affects posterior swim bladder chamber inflation and swimming performance of zebrafish larvae. Aquatic toxicology (Amsterdam, Netherlands). 157C:225-235

Heijlen, M., Houbrechts, A.M., Bagci, E., Van Herck, S.L., Kersseboom, S., Esguerra, C.V., Blust, R., Visser, T.J., Knapen, D., and Darras, V.M. (2014) Knockdown of type 3 iodothyronine deiodinase severely perturbs both embryonic and early larval development in zebrafish. Endocrinology. 155(4):1547-1559

Hagenaars, A., Vergauwen, L., Benoot, D., Laukens, K., and Knapen, D. (2013) Mechanistic toxicity study of perfluorooctanoic acid in zebrafish suggests mitochondrial dysfunction to play a key role in PFOA toxicity. Chemosphere. 91(6):844-56

Selderslaghs, I.W., Hooyberghs, J., Blust, R., and Witters, H.E. (2013) Assessment of the developmental neurotoxicity of compounds by measuring locomotor activity in zebrafish embryos and larvae. Neurotoxicology and teratology. 37:44-56

Vergauwen, L., Knapen, D., Hagenaars, A., and Blust, R. (2013) Hypothermal and hyperthermal acclimation differentially modulate cadmium accumulation and toxicity in the zebrafish. Chemosphere. 91(4):521-529

Vergauwen, L., Hagenaars, A., Blust, R., and Knapen, D. (2013) Temperature dependence of long-term cadmium toxicity in the zebrafish is not explained by liver oxidative stress: Evidence from transcript expression to physiology. Aquatic toxicology (Amsterdam, Netherlands). 126C:52-62

Vergauwen, L., Knapen, D., Hagenaars, A., De Boeck, G., and Blust, R. (2013) Assessing the impact of thermal acclimation on physiological condition in the zebrafish model. Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology. 183(1):109-121

Hagenaars, A., Vergauwen, L., De Coen, W., and Knapen, D. (2011) Structure-activity relationship assessment of four perfluorinated chemicals using a prolonged zebrafish early life stage test. Chemosphere. 82(5):764-772

Vergauwen, L., Benoot, D., Blust, R., and Knapen, D. (2010) Long-term warm or cold acclimation elicits a specific transcriptional response and affects energy metabolism in zebrafish. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 157(2):149-157

Komjarova, I., and Blust, R. (2009) Multimetal interactions between Cd, Cu, Ni, Pb, and Zn uptake from water in the zebrafish Danio rerio. Environmental science & technology. 43(19):7225-7229

De Wit, M., Keil, D., Remmerie, N., Ven, K.V., Brandhof, E.J., Knapen, D., Witters, E., and Coen, W.D. (2008) Molecular targets of TBBPA in zebrafish analysed through integration of genomic and proteomic approaches. Chemosphere. 74(1):96-105