PUBLICATION
Incubation at 32.5°C and above causes malformations in the zebrafish embryo
- Authors
- Pype, C., Verbueken, E., Saad, M.A., Casteleyn, C.R., Van Ginneken, C.J., Knapen, D., Van Cruchten, S.J.
- ID
- ZDB-PUB-150526-4
- Date
- 2015
- Source
- Reproductive toxicology (Elmsford, N.Y.) 56: 56-63 (Journal)
- Registered Authors
- Knapen, Dries, Van Cruchten, Steven, Verbueken, Evy
- Keywords
- Development, Embryo, Hatching enzyme, Malformation, Morphology, Temperature, Zebrafish
- MeSH Terms
-
- Animals
- Biological Assay*
- Cathepsin L/metabolism
- Embryo, Nonmammalian/abnormalities*
- Embryo, Nonmammalian/enzymology
- Temperature*
- Time Factors
- Toxicity Tests/methods*
- Zebrafish/abnormalities*
- Zebrafish/metabolism
- Zebrafish Proteins/metabolism
- PubMed
- 26005098 Full text @ Reprod. Toxicol.
Citation
Pype, C., Verbueken, E., Saad, M.A., Casteleyn, C.R., Van Ginneken, C.J., Knapen, D., Van Cruchten, S.J. (2015) Incubation at 32.5°C and above causes malformations in the zebrafish embryo. Reproductive toxicology (Elmsford, N.Y.). 56:56-63.
Abstract
Zebrafish embryos are increasingly used for developmental toxicity screening of candidate drugs and are occasionally co-incubated with a metabolic activation system at 32°C for 1, 2 or 4h, depending on their developmental stage. As this temperature is higher than the optimal temperature for zebrafish embryonic development (26-28.5°C), we investigated whether continuous incubation of zebrafish embryos from 2.5 until 96hours post fertilization (hpf) at high temperatures (30.5-36.5°C) causes malformations. At 32.5°C tail malformations were observed as early as 24 hpf, and these became even more prominent at 34.5 and 36.5°C. Cardiovascular and head malformations, edema and blood accumulations throughout the body were present at 36.5°C. Finally, temperatures higher than 28.5°C accelerated embryonic development except for 36.5°C, at which a lower hatching rate and hatching enzyme activity were observed. In conclusion, incubation of zebrafish embryos at 32.5°C and above from 2.5 until 96 hpf causes malformations as early as 24 hpf.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping