PUBLICATION
Thyroid Hormone Disruptors Interfere with Molecular Pathways of Eye Development and Function in Zebrafish
- Authors
- Baumann, L., Segner, H., Ros, A., Knapen, D., Vergauwen, L.
- ID
- ZDB-PUB-190403-2
- Date
- 2019
- Source
- International Journal of Molecular Sciences 20(7): (Journal)
- Registered Authors
- Knapen, Dries, Vergauwen, Lucia
- Keywords
- PTU, TBBPA, microarray, mode of action, pathways of toxicity, transcriptome analyses
- Datasets
- GEO:GSE121338, GEO:GSE121336, GEO:GSE121334
- MeSH Terms
-
- Animals
- Down-Regulation/drug effects
- Down-Regulation/genetics
- Endocrine Disruptors/toxicity*
- Eye/drug effects
- Eye/embryology*
- Gene Expression Profiling
- Polybrominated Biphenyls/toxicity
- Propylthiouracil/toxicity
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Thyroid Hormones/toxicity*
- Transcription, Genetic/drug effects
- Up-Regulation/drug effects
- Up-Regulation/genetics
- Zebrafish/metabolism*
- PubMed
- 30934780 Full text @ Int. J. Mol. Sci.
Citation
Baumann, L., Segner, H., Ros, A., Knapen, D., Vergauwen, L. (2019) Thyroid Hormone Disruptors Interfere with Molecular Pathways of Eye Development and Function in Zebrafish. International Journal of Molecular Sciences. 20(7):.
Abstract
The effects of thyroid hormone disrupting chemicals (THDCs) on eye development of zebrafish were investigated. We expected THDC exposure to cause transcriptional changes of vision-related genes, which find their phenotypic anchoring in eye malformations and dysfunction, as observed in our previous studies. Zebrafish were exposed from 0 to 5 days post fertilization (dpf) to either propylthiouracil (PTU), a thyroid hormone synthesis inhibitor, or tetrabromobisphenol-A (TBBPA), which interacts with thyroid hormone receptors. Full genome microarray analyses of RNA isolated from eye tissue revealed that the number of affected transcripts was substantially higher in PTU- than in TBBPA-treated larvae. However, multiple components of phototransduction (e.g., phosphodiesterase, opsins) were responsive to both THDC exposures. Yet, the response pattern for the gene ontology (GO)-class "sensory perception" differed between treatments, with over 90% down-regulation in PTU-exposed fish, compared to over 80% up-regulation in TBBPA-exposed fish. Additionally, the reversibility of effects after recovery in clean water for three days was investigated. Transcriptional patterns in the eyes were still altered and partly overlapped between 5 and 8 dpf, showing that no full recovery occurred within the time period investigated. However, pathways involved in repair mechanisms were significantly upregulated, which indicates activation of regeneration processes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping