ZFIN ID: ZDB-LAB-080703-1
Megason Lab
PI/Director: Megason, Sean
Contact Person: Megason, Sean
Email: megason@hms.harvard.edu
URL: http://www.digitalfish.org/
Address: Department of Systems Biology Harvard Medical School 200 Longwood Blvd - WAB 536 Boston, MA 02115-5730
Country: United States
Phone: 617.432.7441
Fax: 617.432.5012
Line Designation: hm

Show all 19 genomic features

The Megason lab is interested in how the program contained in the genome is executed during development to turn an egg into an embryo. We use confocal/2-photon imaging of living, transgenic zebrafish embryos to watch biological circuits function in vivo and use these data in cell-based, quantitative modeling

Gelas, Arnaud Post-Doc Mosaliganti, Kishore Post-Doc Noche, Ramil Post-Doc
Obholzer, Nikolaus Post-Doc Swinburne, Ian Post-Doc Tentner, Andrea Post-Doc
Cowgill, Paul Graduate Student Xiong, Fengzhu Graduate Student Rannou, Nicolas Technical Staff
Souhait, Lydie Technical Staff

Wang, A., Zhang, Q., Han, Y., Megason, S., Hormoz, S., Mosaliganti, K.R., Lam, J.C.K., Li, V.O.K. (2022) A novel deep learning-based 3D cell segmentation framework for future image-based disease detection. Scientific Reports. 12:342
Munjal, A., Hannezo, E., Tsai, T.Y., Mitchison, T.J., Megason, S.G. (2021) Extracellular hyaluronate pressure shaped by cellular tethers drives tissue morphogenesis. Cell. 184:6313-6325.e18
Tsai, T.Y., Sikora, M., Xia, P., Colak-Champollion, T., Knaut, H., Heisenberg, C.P., Megason, S.G. (2020) An adhesion code ensures robust pattern formation during tissue morphogenesis. Science (New York, N.Y.). 370:113-116
Demy, D.L., Carrère, M., Noche, R., Tauzin, M., Le Bris, M., Baek, C., Leshchiner, I., Goessling, W., Herbomel, P. (2020) The cationic amino acid exporter Slc7a7 is induced and vital in tissue macrophages with sustained efferocytic activity. Journal of Cell Science. 133(20):
Smith, L., Fabian, L., Al-Maawali, A., Noche, R.R., Dowling, J.J. (2020) De novo phosphoinositide synthesis in zebrafish is required for triad formation but not essential for myogenesis. PLoS One. 15:e0231364
Volpatti, J.R., Endo, Y., Knox, J., Groom, L., Brennan, S., Noche, R., Zuercher, W.J., Roy, P., Dirksen, R.T., Dowling, J.J. (2020) Identification of drug modifiers for RYR1 related myopathy using a multi-species discovery pipeline. eLIFE. 9:
Mosaliganti, K.R., Swinburne, I.A., Chan, C.U., Obholzer, N.D., Green, A.A., Tanksale, S., Mahadevan, L., Megason, S.G. (2019) Size control of the inner ear via hydraulic feedback. eLIFE. 8:
O'Brown, N.M., Megason, S.G., Gu, C. (2019) Suppression of transcytosis regulates zebrafish blood-brain barrier function. eLIFE. 8:
Ishimatsu, K., Cha, A., Collins, Z.M., Megason, S.G. (2019) Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling. Journal of visualized experiments : JoVE. (147):
Swinburne, I.A., Mosaliganti, K.R., Upadhyayula, S., Liu, T.L., Hildebrand, D.G.C., Tsai, T.Y., Chen, A., Al-Obeidi, E., Fass, A.K., Malhotra, S., Engert, F., Lichtman, J.W., Kirchausen, T., Betzig, E., Megason, S.G. (2018) Lamellar projections in the endolymphatic sac act as a relief valve to regulate inner ear pressure. eLIFE. 7
Ishimatsu, K., Hiscock, T.W., Collins, Z.M., Sari, D.W.K., Lischer, K., Richmond, D.L., Bessho, Y., Matsui, T., Megason, S.G. (2018) Size-reduced embryos reveal a gradient scaling based mechanism for zebrafish somite formation. Development (Cambridge, England). 145(11)
Briggs, J.A., Weinreb, C., Wagner, D.E., Megason, S., Peshkin, L., Kirschner, M.W., Klein, A.M. (2018) The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution. Science (New York, N.Y.). 360(6392)
Wagner, D.E., Weinreb, C., Collins, Z.M., Briggs, J.A., Megason, S.G., Klein, A.M. (2018) Single-cell mapping of gene expression landscapes and lineage in the zebrafish embryo. Science (New York, N.Y.). 360(6392):981-987
Hiscock, T.W., Miesfeld, J.B., Mosaliganti, K.R., Link, B.A., Megason, S.G. (2018) Feedback between tissue packing and neurogenesis in the zebrafish neural tube. Development (Cambridge, England). 145(9)
Liu, T.L., Upadhyayula, S., Milkie, D.E., Singh, V., Wang, K., Swinburne, I.A., Mosaliganti, K.R., Collins, Z.M., Hiscock, T.W., Shea, J., Kohrman, A.Q., Medwig, T.N., Dambournet, D., Forster, R., Cunniff, B., Ruan, Y., Yashiro, H., Scholpp, S., Meyerowitz, E.M., Hockemeyer, D., Drubin, D.G., Martin, B.L., Matus, D.Q., Koyama, M., Megason, S.G., Kirchhausen, T., Betzig, E. (2018) Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms. Science (New York, N.Y.). 360(6386):
Green, A.A., Mosaliganti, K.R., Swinburne, I.A., Obholzer, N.D., Megason, S.G. (2017) Recovery of shape and size in a developing organ pair. Developmental Dynamics : an official publication of the American Association of Anatomists. 246:451-465
Aguet, F., Upadhyayula, S., Gaudin, R., Chou, Y.Y., Cocucci, E., He, K., Chen, B.C., Mosaliganti, K., Pasham, M., Skillern, W., Legant, W.R., Liu, T.L., Findlay, G., Marino, E., Danuser, G., Megason, S., Betzig, E., Kirchhausen, T. (2016) Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy. Molecular biology of the cell. 27(22):3418-3435
Nissim, S., Weeksb, O., Talbot, J.C., Hedgepeth, J.W., Wucherpfennig, J., Schatzman-Bone, S., Swinburne, I., Cortes, M., Alexa, K., Megason, S., North, T.E., Amacher, S.L., Goessling, W. (2016) Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development. Developmental Biology. 418(1):108-23
Lee, S.G., Huang, M., Obholzer, N.D., Sun, S., Li, W., Petrillo, M., Dai, P., Zhou, Y., Cotanche, D.A., Megason, S.G., Li, H., Chen, Z.Y. (2016) Myc and Fgf Are Required for Zebrafish Neuromast Hair Cell Regeneration. PLoS One. 11:e0157768
Swinburne, I.A., Mosaliganti, K.R., Green, A.A., Megason, S.G. (2015) Improved Long-Term Imaging of Embryos with Genetically Encoded α-Bungarotoxin. PLoS One. 10:e0134005
Xiong, F., Megason, S.G. (2015) Abstracting the principles of development using imaging and modeling. Integrative biology : quantitative biosciences from nano to macro. 7(6):633-42
Xiong, F., Obholzer, N.D., Noche, R.R., Megason, S.G. (2015) Multibow: Digital Spectral Barcodes for Cell Tracing. PLoS One. 10:e0127822
Stooke-Vaughan, G.A., Obholzer, N.D., Baxendale, S., Megason, S.G., Whitfield, T.T. (2015) Otolith tethering in the zebrafish otic vesicle requires Otogelin and α-Tectorin. Development (Cambridge, England). 142(6):1137-45
Hiscock, T.W., Megason, S.G. (2015) Mathematically guided approaches to distinguish models of periodic patterning. Development (Cambridge, England). 142:409-19
Xiong, F., Ma, W., Hiscock, T.W., Mosaliganti, K.R., Tentner, A.R., Brakke, K.A., Rannou, N., Gelas, A., Souhait, L., Swinburne, I.A., Obholzer, N.D., Megason, S.G. (2014) Interplay of Cell Shape and Division Orientation Promotes Robust Morphogenesis of Developing Epithelia. Cell. 159:415-427
Mosaliganti, K.R., Gelas, A., and Megason, S.G. (2013) An efficient, scalable, and adaptable framework for solving generic systems of level-set PDEs. Frontiers in Neuroinformatics. 7:35
Xiong, F., Tentner, A.R., Huang, P., Gelas, A., Mosaliganti, K.R., Souhait, L., Rannou, N., Swinburne, I.A., Obholzer, N.D., Cowgill, P.D., Schier, A.F., and Megason, S.G. (2013) Specified neural progenitors sort to form sharp domains after noisy shh signaling. Cell. 153(3):550-561
Miller, A.C., Obholzer, N.D., Shah, A.N., Megason, S.G., and Moens, C.B. (2013) RNA-seq based mapping and candidate identification of mutations from forward genetic screens. Genome research. 23(4):679-686
Obholzer, N., Swinburne, I.A., Schwab, E., Nechiporuk, A.V., Nicolson, T., and Megason, S.G. (2012) Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing. Development (Cambridge, England). 139(22):4280-4290
Xu, C., Fan, Z.P., Müller, P., Fogley, R., Dibiase, A., Trompouki, E., Unternaehrer, J., Xiong, F., Torregroza, I., Evans, T., Megason, S.G., Daley, G.Q., Schier, A.F., Young, R.A., and Zon, L.I. (2012) Nanog-like Regulates Endoderm Formation through the Mxtx2-Nodal Pathway. Developmental Cell. 22(3):625-238
Lin, Y.F., Swinburne, I., and Yelon, D. (2012) Multiple influences of blood flow on cardiomyocyte hypertrophy in the embryonic zebrafish heart. Developmental Biology. 362(2):242-253
Huang, P., Xiong, F., Megason, S.G., and Schier, A.F. (2012) Attenuation of notch and hedgehog signaling is required for fate specification in the spinal cord. PLoS Genetics. 8(6):e1002762
Mosaliganti, K.R., Noche, R.R., Xiong, F., Swinburne, I.A., and Megason, S.G. (2012) ACME: Automated Cell Morphology Extractor for Comprehensive Reconstruction of Cell Membranes. PLoS Computational Biology. 8(12):e1002780
Trinh, A., Hochgreb, T., Graham, M., Wu, D., Ruf-Zamojski, F., Jayasena, C.S., Saxena, A., Hawk, R., Gonzalez-Serricchio, A., Dixson, A., Chow, E., Gonzales, C., Leung, H.Y., Solomon, I., Bronner-Fraser, M., Megason, S.G., and Fraser, S.E. (2011) A versatile gene trap to visualize and interrogate the function of the vertebrate proteome. Genes & Development. 25(21):2306-20
Agapakis, C.M., Niederholtmeyer, H., Noche, R.R., Lieberman, T.D., Megason, S.G., Way, J.C., and Silver, P.A. (2011) Towards a synthetic chloroplast. PLoS One. 6(4):e18877
Sheets, L., Trapani, J.G., Mo, W., Obholzer, N., and Nicolson, T. (2011) Ribeye is required for presynaptic CaV1.3a channel localization and afferent innervation of sensory hair cells. Development (Cambridge, England). 138(7):1309-1319
Noche, R.R., Lu, P.N., Goldstein-Kral, L., Glasgow, E., and Liang, J.O. (2011) Circadian rhythms in the pineal organ persist in zebrafish larvae that lack ventral brain. BMC Neuroscience. 12:7
Wühr, M., Obholzer, N.D., Megason, S.G., Detrich, H.W., and Mitchison, T.J. (2011) Live imaging of the cytoskeleton in early cleavage-stage zebrafish embryos. Methods in cell biology. 101:1-18
Trapani, J.G., Obholzer, N., Mo, W., Brockerhoff, S.E., and Nicolson, T. (2009) Synaptojanin1 is required for temporal fidelity of synaptic transmission in hair cells. PLoS Genetics. 5(5):e1000480
Megason, S.G. (2009) In toto imaging of embryogenesis with confocal time-lapse microscopy. Methods in molecular biology (Clifton, N.J.). 546:317-332
Pierce, L.X., Noche, R.R., Ponomareva, O., Chang, C., and Liang, J.O. (2008) Novel functions for Period 3 and Exo-rhodopsin in rhythmic transcription and melatonin biosynthesis within the zebrafish pineal organ. Brain research. 1223:11-24
Obholzer, N., Wolfson, S., Trapani, J.G., Mo, W., Nechiporuk, A., Busch-Nentwich, E., Seiler, C., Sidi, S., Söllner, C., Duncan, R.N., Boehland, A., and Nicolson, T. (2008) Vesicular glutamate transporter 3 is required for synaptic transmission in zebrafish hair cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 28(9):2110-2118
Megason, S.G. and Fraser, S.E. (2003) Digitizing life at the level of the cell: high-performance laser-scanning microscopy and image analysis for in toto imaging of development. Mechanisms of Development. 120(11):1407-1420