PUBLICATION
Multibow: Digital Spectral Barcodes for Cell Tracing
- Authors
- Xiong, F., Obholzer, N.D., Noche, R.R., Megason, S.G.
- ID
- ZDB-PUB-150527-4
- Date
- 2015
- Source
- PLoS One 10: e0127822 (Journal)
- Registered Authors
- Megason, Sean, Noche, Ramil, Obholzer, Nikolaus, Xiong, Fengzhu
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cells/cytology*
- Cloning, Molecular
- Developmental Biology/methods*
- Fluorescent Antibody Technique/methods*
- Image Processing, Computer-Assisted
- Microscopy, Confocal
- Molecular Typing/methods*
- Specimen Handling/methods*
- Staining and Labeling/methods
- Zebrafish
- PubMed
- 26010570 Full text @ PLoS One
Citation
Xiong, F., Obholzer, N.D., Noche, R.R., Megason, S.G. (2015) Multibow: Digital Spectral Barcodes for Cell Tracing. PLoS One. 10:e0127822.
Abstract
We introduce a multicolor labeling strategy (Multibow) for cell tracing experiments in developmental and regenerative processes. Building on Brainbow-based approaches that produce colors by differential expression levels of different fluorescent proteins, Multibow adds a layer of label diversity by introducing a binary code in which reporters are initially OFF and then probabilistically ON or OFF following Cre recombination. We have developed a library of constructs that contains seven different colors and three different subcellular localizations. Combining constructs from this library in the presence of Cre generates cells labeled with multiple independently expressed colors based on if each construct is ON or OFF following recombination. These labels form a unique "barcode" that allows the tracking of the cell and its clonal progenies in addition to expression level differences of each color. We tested Multibow in zebrafish which validates its design concept and suggests its utility for cell tracing applications in development and regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping