Lechermeier et al., 2019 - Transcript Analysis of Zebrafish GLUT3 Genes, slc2a3a and slc2a3b, Define Overlapping as Well as Distinct Expression Domains in the Zebrafish (Danio rerio) Central Nervous System. Frontiers in molecular neuroscience   12:199 Full text @ Front. Mol. Neurosci.

Figure 2 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 2-cell to Adult

Figure 3

Whole mount RNA in situ hybridization of slc2a3a at early embryonic stages [18–36 hours post fertilization (hpf)]. Pictures in the left column depict alternating lateral (A,C,E,G) and dorsal (B,D,F,H) views. Anterior is to the left. Higher magnifications (a1–h3) corresponding to boxes in (A–H). Arrowheads indicate examples of labeled cells or cell populations. Note staining in spinal cord, hindbrain, ventral midbrain, ventral thalamus, ventral telencephalon and epiphysis. Two bilateral rows, one medially (black arrowheads) and one laterally (white arrowheads) of positive cells are situated in the ventral hindbrain along the floor plate (f2 and h2). For abbreviations of anatomical terms see Table 1. Scale bars in (A), 100 μm and pertains to (A–H); scale bar in a1, 50 μm and pertains to a1–h3.

Figure 4

Whole mount RNA in situ hybridization of slc2a3a at late embryonic and early post-hatching stages (48–120 hpf). Pictures in the left column depict alternating lateral (A,C,E) and dorsal (B,D,F) views. Anterior is to the left. Higher magnifications (a1–f3) corresponding to boxes in (A–F). Note staining in hindbrain, ventral midbrain, optic tectum, ventral thalamus, ventral telencephalon and retina. Detailed descriptions can be found in the text, for abbreviations see Table 1. Scale bar in (A), 100 μm and pertains to (A–F); scale bar in a1, 50 μm and pertains to a1–f2, d3, f3 and scale bar in a3, 50 μm and pertains to a3, b3.

Figure 5

Cryosections (20 μm) of 120 hpf embryo processed for RNA in situ hybridization for slc2a3a. The sections (A–P) are cross sections arranged from anterior to posterior at the levels indicated in Figure 4E. Detailed descriptions can be found in the text, for abbreviations see Table 1. Scale bar, 100 μm.

Figure 6

Whole mount RNA in situ hybridization for slc2a3b at blastula 128-cell, 24 hpf and 72 hpf stages. For comparison, a sense probe was included (B,E). Comparison of embryos incubated with antisense (A,A’,C,D) and sense (B,B’,E) show diffuse staining only in embryos incubated with the antisense probe. Pictures (F; lateral) and (G; dorsal) show 72 hpf larvae stained with antisense probe. Boxes in (F) and (G) depict locations of high magnification pictures in (F’,G’). For abbreviations see Table 1. Scale bars in (A,C,D), 100 μm and pertains to (A–G), respectively, scale bar in (A’,F’), 50 μm and pertains to (A’,B’,F’,G’), respectively.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 128-cell to Protruding-mouth

Figure 7

RNA in situ hybridization of slc2a3a in the adult brain. Pictures (A–R) show cross sections of an adult zebrafish brain (80 μm) from anterior to posterior as indicated in the scheme. (B’–R’) are high magnifications of boxed areas in (B–R). Detailed descriptions can be found in the text, for abbreviations see Table 1. Scale bar in (A), 100 μm and pertains to (A–R); scale bars in (B’–R’), 20 μm.

Figure 8

RNA in situ hybridization of adult expression of slc2a3b. Pictures show cross sections of an adult zebrafish brain (80 μm) from anterior to posterior as indicated in the scheme. Panels (A’–J’) are high magnifications of boxed areas in (A–J). Black arrowheads in (A’,B’,J’) indicate example of single-positive cells. Detailed descriptions can be found in the text, for abbreviations see Table 1. Scale bar in (A), 100 μm and pertains to (A–J); scale bars in (A’–J’), 20 μm.

Figure 9

RNA in situ hybridization of gad1b and double RNA in situ hybridization for slc2a3a and gad1b, a marker for GABAergic neurons. For comparison, a single in situ hybridization for gad1b was performed at 24 hpf (A,B,B’) and 36 hpf (C,D,D’) old zebrafish embryos. Boxes in (A,B,B’,C,D,D’) depict location of high magnification pictures a1,a2,b1,b2 and c1, c2, d1, d2, respectively. In addition, a 24 hpf old zebrafish embryo was stained with slc2a3a DIG in situ probe (blue) and gad1b FLUO probe (red). Upper rows show lateral views (A,C,E) and lower rows ventral views (B,B’,D,D’,F). Anterior is to the left. Boxes in (E,F) depict location of high magnification pictures e1–e4 and f1–f4, respectively. Boxes in e1 and e2 and f1, f2, and f4) represent higher magnification pictures shown in e1’, e2’ and f1’, f2’, and f4’. Box in e1’ represents location of even higher magnification shown in e1”. White arrows mark double-positive cells or cell populations. Red arrow marks an example of cells only positive for gad1b and blue arrows cells only positive for slc2a3a. Scale bars in (A,E), 100 μm and pertains to (A–F); scale bar in a1, 50 μm and valid for a1–d2; scale bar in e1, 50 μm and pertains to e1–e2 and f1–2; scale bar in e3, 50 μm and valid for e3-e4, and f3–4, scale bars in e1’–f4’, e1’, 10 μm.

Acknowledgments:
ZFIN wishes to thank the journal Frontiers in molecular neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Mol. Neurosci.